bromphenol blue


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brom·phe·nol blue

(brom-fē-nol blū),
A substituted triphenylmethane dye (MW 670, pK 4.0), used as an acid-base indicator (yellow at pH less than 3.1, blue at pH more than 4.7); also used for histochemical and electrophoretic demonstration of proteins.
Synonym(s): bromophenol blue
References in periodicals archive ?
Afterward, beads were washed twice with lysis buffer, and 40 [micro]L of 2x sample buffer (100 mmol/L Tris, pH 6.80; 40 g/L SDS; 20 mL/L glycerol; 20 /[micro]g/L bromphenol blue; 15 g/L dithiothreitol) was added to each tube.
We prepared samples for electrophoresis by adding 20 [micro]L of sample buffer (40 mmol/L Tris, 33 g/L SDS, 500 mL/L glycerol, and bromphenol blue) to 80 [micro]L of eluate.
The amplicons were mixed with equal amounts of single-strand conformation polymorphism (SSCP) buffer containing 950 mL/L formamide, 10 mmol/L NaOH, 2.5 g/L bromphenol blue, and 2.5 g/L xylene cyanol and then submitted to mutation screening by SSCP and heteroduplex analysis.
We then added 110 [micro]L of rehydration solution (7 mol/L urea, 2 mol/L thiourea, 40 g/L CHAPS, 60 mmol/L DTT, 5 mL/L IPG buffer pH 3-10 NL, and a trace of bromphenol blue) to the above mixture.
Gelatinase calibrators were prepared by diluting healthy capillary blood with 15 volumes of nonreducing sample buffer (containing, per liter, 62.5 mmol of Tris-HCl, pH 6.8; 350 mL of glycerol; 25 mL of Triton X-100; 40 g of sodium dodecyl sulfate; 0.2 g of Brij-35; 0.02 g of Na[[N.SUB.3]; and 0.1 g of bromphenol blue) (34).
We denatured 20 [micro]L of sample, containing 5-50 ng of protein, by heating in sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis sample buffer [50 mmol/L Tris-HCl (pH 6.8), 0.1 mmol/L dithiothreitol, 20 g/L SDS, 1 g/L bromphenol blue, 100 g/L glycerol] at 75[degrees]C for 10 min and run on a 4-12% polyacrylamide/ SDS gel (Invitrogen).
We diluted 1 [micro]L of human EDTA plasma that had been stored for up to 2 years at -70 [degrees]C in 20 [micro]L of sample loading buffer [10 g/L sodium dodecyl sulfate, 100 mL/L glycerol, 25 mmol/L Tris (pH 6.8), 0.05 g/L bromphenol blue, and 50 mL/L ([beta]-mercaptoethanol] and boiled the mixture for 5 min.
For SSCP and heteroduplexes analysis, PCR products were mixed with equal amounts of SSCP buffer containing 950 mL/L formamide, 10 mmol/L NaOH, 2.5 g/L bromphenol blue, and 2.5 g/L xylene cyanol.
A 2-[micro]L portion of the PCR product was diluted with 4 [micro]L of the SSCP buffer (475 mL/L formamide, 0.5 g/L xylene cyanol, 0.2 g/L bromphenol blue, 5 mmol/L Tris, and 0.5 mmol/L EDTA).
Ten microliters each of the PCR product and the loading buffer (980 mL/L formamide, 0.1 g/L bromphenol blue, 0.1 g/L xylene cyanol, and 10 mmol/L EDTA) were mixed and boiled in water for 5 min.