Bromphenol blue | definition of bromphenol blue by Medical dictionary
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brom·phe·nol blue (brom-fē-nol blū),
A substituted triphenylmethane dye (MW 670, pK 4.0), used as an acid-base indicator (yellow at pH less than 3.1, blue at pH more than 4.7); also used for histochemical and electrophoretic demonstration of proteins.
References in periodicals archive
We also validated the method by assaying several serum samples with known autoantibody profiles in separate wells, and we added bromphenol blue
to the spotting solutions to monitor for any failure in probe deposition.
PTMs did not stain with bromphenol blue
for protein, and a Bradford assay indicated that only about 3.
Serum (1 [micro]L with bromphenol blue
added as a visual marker) was separated by electrophoresis (12 g/L agarose, pH 8.
1 mmol/L dithiothreitol, 20 g/L SDS, 1 g/L bromphenol blue
, 100 g/L glycerol] at 75[degrees]C for 10 min and run on a 4-12% polyacrylamide/ SDS gel (Invitrogen).