Number The name of reagent [R.sub.1] [R.sub.2] [R.sub.3] I Bromocresol green Br Br C[H.sub.3] II Bromophenol blue
H Br Br in Bromothymol blue Br CH(C[H.sub.3]) C[H.sub.3] IV Bromocresol purple Br C[H.sub.3] H V Bromocresol blue Br C[H.sub.3] Br VI o-Cresol red C[H.sub.3] H H VII p-Cresol blue H C[H.sub.3] H VIII m-Cresol purple H H C[H.sub.3]
In the assays with bromophenol blue
, the long tailing phenomenon is less evident because is released faster than eosin Y, which probably remains adsorbed in the support medium, being discharged slowly in the effluent of the reactor.
is a tetrabromophenol sulfonaphthalein, widely used as a 'tracking dye' by the scientific community.
In the histochemical characterization of EFNs, a positive reaction of ferric chloride (Figure 2b), zinc chloride iodine (Figure 2c), bromophenol blue
(Figure 2d), Periodic Acid Schiff (Figure 2e) and Fehling (Figure 2f) revealed the presence of phenolic compounds, starch, proteins, neutral polysaccharides and reducing sugars, respectively.
Protein pellet was reconstituted in rehydration buffer (8 M urea, 2% CHAPS, 0.2% DTT (dithiothreitol), 0.5% IPG buffer pH 3-10, and 0.002% bromophenol blue
) and the concentration was determined.
The digestion step was done at 5[degrees]C for 90 minutes and stopped immediately by adding 5 [micro]L of 0.225 EDTA and 2 [micro]L of bromophenol blue
loading dye per sample and mixing thoroughly.
Same amounts of protein were then diluted in SDS sample buffer (Tris-HCl, glycerol, SDS, DTT, and bromophenol blue
), subjected to 10% SDS-PAGE, and immunoblotted with antibodies specific for AMPKa, phospho-AMPK-[alpha] (Thr172) (Cell Signaling Technology).
For SDS-PAGE, the serum samples were diluted in phosphate buffer (pH 7.2) and proteins were denatured by heating with loading dye (1.54 g dithiothreitol, 2 g sodiumdodocyle sulfate, 8 mL of 1.0 M Tris HCl; pH 6.8, 10 mL of glycerol and 20 mg of bromophenol blue
dye) in boiling water bath for two minutes before loading on the gel.
Electrophoresis--10pL of PCR products were mixed to 1.5pL of blue juice (0.01% bromophenol blue
, 40% glycerol).
Generally this will also contain one or more negatively charged, large molecule dyes like bromophenol blue
or xylene cyanol, which will help us see the sample as we load it in the well, and serve as a tracking dye.
Zymography: To analyze [MMP.sub.s] , 20 mg of total sperm protein and medium (sperm cultured medium) were mixed with 2 [micro]l/10 [micro]l FOZ loading buffer (5% bromophenol blue
, 10% SDS, 2% glycerol) and reacted on ice for 5 min.
A 10 [micro]L reaction solution was mixed with 1 [micro]L of loading buffer (50% [v/v] glycerol, 0.9% [w/v] SDS, and 0.05% [w/v] bromophenol blue
) and electrophoresed on a 1.5% agarose gel in 1 x TBE buffer.