The hydrodynamic assays carried out with bromophenol blue
presented advance in the tracer response of approximately 40, 43 and 7% of the real [[theta].
F-cells stained positively in a darker blue with Mercuric Bromophenol Blue
caused by the presence of proteins (Fig.
It can be observed that the edges of the lipid bodies react positively to the Bromophenol Blue
, indicating the presence of proteins in this region of the droplet (Figure 2).
The system was run at 5 W per gel for the first 30 min followed by 12 W per gel for 6-7 h until the bromophenol blue
line reached the bottom of the gels in the Ettan-Dalt II system (Amersham Pharmacia, San Francisco, CA, USA).
05% (w/v) bromophenol blue
and xylene cyanol FF] were added to each PCR product and 5[micro]L of each was loaded in the gel.
Tissue sections (5 [micro]m) were stained with hematoxylin and eosin (Junqueira & Junqueira, 1983) and with bromophenol blue
for detection of proteins (Pearse 1960).
4% bromophenol blue
, 10% [Beta]-mercaptoethanol) and boiled for 5 min.
Retention factor was calculated based on the migration of protein per the migration of bromophenol blue
0625M), SDS (2%), Mecaptoethanol (5%), Glycerol (10%), Bromophenol blue
base, 2 ml of 10% SDS, 1 ml Glycerol, 2 mg Bromophenol blue
, 25 [micro]l 2Mercaptoethanol).
Electrophoresis was carried out at constant current of 2 mA/cm using electrode buffer until the bromophenol blue
marker head reached the bottom of the gel.
25% of bromophenol blue
and 60% of glycerol) was added to the each sample, which were analyzed by electrophoresis (4h at 90 V) in agarose gel (1.