The prognostic value of proliferation indices: a study with in vivo bromodeoxyuridine
At the end of the incubation, bromodeoxyuridine
was added to a final concentration of 30 [micro]mol/L, and the cell culture was incubated for 60 min at 37[degrees]C under 95% air-5% C[O.
1) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide (MTT) assay and bromodeoxyuridine
To determine cell proliferation, we added bromodeoxyuridine
(BrdU; 30 [micro]g/mL; 1%; Amersham Biosciences, Little Chalfont, Bucks, England) during the last 3 hr of culture.
The functional relationship between in vivo bromodeoxyuridine
labeling index and Ki-67 proliferation index in human breast cancer.
Thermal denaturation of DNA from bromodeoxyuridine
Dioscorea composita or Increased bromodeoxyuridine
Dioscorea villosa uptake and intracellular cAMP L (Dioscoreaceae) level in keratinocytes 14.
A comparison of the predictive power for survival in gtiomas provided by MIB-1, bromodeoxyuridine
and proliferating cell nuclear antigen with histopathologic and clinical parameters.
UV-irradiated cells and non-irradiated cells were labeled with 20 mM bromodeoxyuridine
(BrdU; Amersham, Buckinghamshire, UK) for 10 min in 15-mL conical tubes, and then harvested and fixed overnight at 4[degrees]C with 70% ethanol.
(BrdU) was injected in vivo to label the proliferating cells at hippocampus, and morphometry was used to count the hippocampal neurons.
2005); b) by measurement of the incorporation of bromodeoxyuridine
(BrdU), which was added to the incubation media for the last 4 hr of culture, and c) by measurement of testosterone secreted into the culture media over the 48-hr culture period.
Phytoheamagglutinin (PHA-P), Hoechst 33258, Bromodeoxyuridine
(BrdU) and colchicine were obtained from Sigma Chemicals, USA.