bromodeoxyuridine


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bro·mo·de·ox·y·ur·i·dine (BrDu),

(brō'mō-dē-ok'sē-yūr'i-dēn),
A compound that competes with uridine for incorporation in RNA and fluoresces in ultraviolet light; used in BrDu-banding.

bromodeoxyuridine

A thymidine analogue which induces point mutations by tautomerisation. It is incorporated into DNA during the S-phase of the cell cycle, and used to study DNA replication, DNA synthesis by flow cytometry, and cell cycle and tumour kinetics.
References in periodicals archive ?
Immunocytochemical detection of bromodeoxyuridine in proliferating cells of regenerating Cuvierian tubules of Holothuria forskali.
To label bone marrow-derived MSCs in vitro, 10 [micro]l of bromodeoxyuridine (BrdU) solution (1mM BrdU in 1X Dulbecco's PBS, BD Pharmingen) was carefully added for each ml of tissue culture medium.
Dose response of hepatic and renal DNA synthetic rates to continuous exposure to bromodeoxyuridine (BrdU) via slow-release pellets or osmotic minipumps im male B6C3F1 mice.
Image analysis of bromodeoxyuridine (BrdU) staining for the measurement of S-phase in rat and mouse liver.
Similarly, previous results in Brachyhypopomus gauderio, using bromodeoxyuridine as the cell marker, demonstrated an increase in cell proliferation across the brain in individuals maintained in a wild environment compared with the individuals maintained in captivity (13).
Bromodeoxyuridine labeling index as an indicator of early tumor response to preoperative radiotherapy in patients with rectal cancer.
T cell proliferative response was assessed against bovine collagen II by measuring incorporation of bromodeoxyuridine into deoxyribonucleic acid of proliferating cells and by expression of CD25 on proliferating cells as percentage of CD3+/bromodeoxyuridine+ and CD3+/CD25+ T-cells, respectively.
kinetics of SMC1 phosphorylation, (6) incorporation of postradiation bromodeoxyuridine to assess the integrity of the S-phase control point, (7) monoubiquitination of the Fanconi D2 protein, (8) expression of the ATM protein, and (9) expression and function of nonhomologous DNA- binding proteins.
Cell proliferation was indicated by bromodeoxyuridine (BrdU) incorporation analysis using an enzyme-linked immunosorbent assay (ELISA) kit (Millipore Corporation, Billerica, MA, USA).
Larsen, "Chapter 23 Washless double staining of a nuclear antigen (Ki-67 or bromodeoxyuridine) and DNA in unfixed nuclei," Methods in Cell Biology, vol.
Measurement of cell kinetics in human tumours in vivo using bromodeoxyuridine incorporation and flow cytometry.