Influence of bromodeoxyuridine
radiosensitization on maligmant glioma patient survival: a retrospective comparasion of survival from the Northern California Oncology Group (NCOG) and Ratiation Therapy Oncology Group trials (RTOG) for glioblastoma multiforme and anaplasic astrocytoma [abstract].
Hemocyte proliferation was assessed using incorporation of bromodeoxyuridine
(BrdU, Roche Diagnostic Corp.
Cell-cycle analysis with use of Hoechst 33258 and propidium iodide or with incorporation of bromodeoxyuridine
is briefly described.
As in most recent NSC experiments, the chemical market bromodeoxyuridine
(BrdU) was used to measure cell proliferation (see the sidebar "Measuring Cell Proliferation").
To quantify the rate o f vascular cell proliferation, CL were obtained from ewes that were injected with bromodeoxyuridine
(BrdU; a thymidine analog) 1h before slaughter on days 4, 8, 12, and 15 of their respective estrous cycle.
The chemicals, such as bromodeoxyuridine
, or BrdU, become part of newly formed DNA when dividing cells copy their genetic information.
To enable detection of the transplanted cells, the cell cultures were labeled with a tracking marker bromodeoxyuridine
(BrdU) which incorporates into actively dividing cells, prior to transplantation, a process which had no apparent effect on the growth or engraftment of the cells.
For instance, using bromodeoxyuridine
(BrdU) labeling to assess cell proliferation at PND8, it was found that RSV could protect the neonatal DG, reversing the EtOH-induced reduction in cell proliferation.
Cell proliferation was measured by the bromodeoxyuridine
(BrdU) incorporation assay as described previously (Liu et at.
BrdC is converted to bromodeoxyuridine
(BrdU) in cells and detected by immunohistochemical labeling.
Approximately 50 cells are labeled with tritiated thymidine or its analogue, bromodeoxyuridine
, for each cell in mitosis.
Histological evaluation revealed that bromodeoxyuridine