Human serum albumin, Cohn fraction V, solution, 10 g/dL, was purchased from MP Biomedicals (Irvine, California); bromocresol green
(BCG) sodium salt and bromocresol purple (BCP) free acid were obtained from JT Baker Company (Phillipsburg, New Jersey) and Fluka Company (St.
Relative dye-binding capacity of albumin from several species with HABA, bromocresol green
and Spectru AB-2.
Blood urea nitrogen, glucose, albumin, creatinine and total protein concentrations were analyzed using an automatic blood analyzer (Express Plus, Ciba-Corning, CA, USA) according to the urease method of Rocch-Ramel (1967), the hexokinase method of Farrance (1987), the bromocresol green
method of Doumas et al.
Sultan, Extraction spectrophotometric determination of mercury (II) using Thiacrown ethers and Bromocresol Green
, Talanta, 1994, 42, 1349-1354.
45 g/l) concentrations were measured by Bromocresol Green
reagent and turbidimetric immunoassay technique (Abbott Architect[R] c16000 automated analyser), respectively.
Bovine serum albumin (BSA), copper(II)ulphate, potassium iodide, cholesterol colour reagent, cholesterol standard, vanillin, alloxan and bromocresol green
are products of sigma chemical company Mo, USA while chloroform, ethanol, phosphoric acid, sulphuric acid, glacial acetic acid and ferric chloride were purchased from BDH chemicals Poole, England, olive oil is a product of May and Baker.
Doumas BT and NG Briggs Serum albumin by bromocresol green
(BCG) method was used to determine serum albumin concentration.
Serum albumin was measured using the bromocresol green
Serum globulins contribute to the discrepancies observed between the bromocresol green
and bromocresol purple assays of serum albumin concentration.