One milliliter of extract solution, 5 mL of phosphate buffer, and 5 mL of bromocresol green
solution were placed in separation and then the solution was mixed well.
Number The name of reagent [R.sub.1] [R.sub.2] [R.sub.3] I Bromocresol green
Br Br C[H.sub.3] II Bromophenol blue H Br Br in Bromothymol blue Br CH(C[H.sub.3]) C[H.sub.3] IV Bromocresol purple Br C[H.sub.3] H V Bromocresol blue Br C[H.sub.3] Br VI o-Cresol red C[H.sub.3] H H VII p-Cresol blue H C[H.sub.3] H VIII m-Cresol purple H H C[H.sub.3]
Albumin levels were measured using the bromocresol green
(BCG) method (Beckman Coulter, Inc., USA).
(2008) by the reaction of alkaloids with bromocresol green
(BCG) forming yellow complex which is extractable by chloroform.
Albumin standards and management of serum albumin with bromocresol green
. Clinica Chimica Acta, 31: 87-96.
Hemoglobin (flow cytometry, Sysmex XN-10[R], Sysmex Corp.), Hb [A.sub.1c] (ion-exchange HPLC, National Glycohemoglobin Standardization Program certified, G7 HPLC analyzer, Tosoh Corp.), fasting glucose (hexokinase), creatinine (compensated kinetic Jaffe with rateblanking), albumin (bromocresol green
), and fructosamine (nitrotetrazolium blue) were measured (Modular[R] P analyzer, Roche Diagnostics).
Albumin was performed by colorimetric (Bromocresol green
) method on Selectra (Merck).
Human serum albumin, Cohn fraction V, solution, 10 g/dL, was purchased from MP Biomedicals (Irvine, California); bromocresol green
(BCG) sodium salt and bromocresol purple (BCP) free acid were obtained from JT Baker Company (Phillipsburg, New Jersey) and Fluka Company (St.
Nitrogen evolved as ammonia was collected in a receiver containing boric acid (2 Percent) solution and mixed indicator (Bromocresol green
and methyl red) and titrated against standard (0.1N) H2So4.
Albumin was estimated by bromocresol green
method and normal range was 3.4-4.8g/dL.
Albumin was determined using the Bromocresol Green
method; albumin binds with Bromocresol Green
in an acidic medium to form a blue-green complex whose colour is proportional to the concentration of albumin in the serum .
Albumin standards and measurement of serum-albumin with bromocresol green
. Clinica Chimica Acta, 31(1): 87-96.