In the VTA (Figure 5), the point of the needle was positioned in the middle of the area, extending from 4.80 to 5.04 mm posterior to bregma
. Two rats of the VTA group did not survive surgery, and eight rats were discarded because the lesion reached other areas.
A 2 mm coronal section (+0.8 mm to -1.2 mm relative to the bregma
) from the ipsilateral hemisphere was homogenized in ice-cold RIPA buffer (Thermo Fisher Scientific, Waltham, MA).
Figure 2A shows representative images of lateral ventricles of PND14 male air-treated (Figure 2A) and CAPs-treated (Figure 2B) animals at similar bregma
. CAPs exposure did not affect the aqueduct of Sylvius (Figure 2E).
separating the frontal from the parietal behind the horn cores is well represented.
All animals were anaesthetized with ketamine and xilasine (112.5 mg/kg and 22.5 mg/kg I.P.) and were stereotaxically implanted (unilaterally) with guide cannulas for injection in the nucleus accumbens shell (coordinates AP + 1.52 mm and ML -0.7 mm relative to bregma
, DV -6.0 mm relative to dura mater) according to Paxinos and Watson's Atlas (1998).
El punto Rolandico superior corresponde al sitio donde el surco central se encuentra con la fisura interhemisferica y se situa en la superficie craneal a 5 centimetros posterior al Bregma
Cortical tissue samples were obtained by inserting a 24-gauge needle 5 mm into the skull bone (IV = 5) through a burr hole craniectomy (2 mm apart from the bregma
MPTPHCl (100 [micro]g/l of saline/side) was bilaterally infused using 5 [micro]L microlitre syringe at the following coordinates of SNc: anteroposterior (AP): -5.0 mm from bregma
; mediolateral (ML): [+ or -] 2.1 mm from midline; dorsoventral (DV): -7.7 mm from skull.
Cortical tissue samples were obtained by means of placing a 22-gauge needle inserted 5 mm in depth into the skull bone (N = 5) through a burr hole craniectomy (2 mm apart from the bregma
) at 24, 48, and 72 hrs.
A[beta] (2535) was administered at the bregma
point with a 50 [micro]L Hamilton microsyringe using a 26-gauge needle that was inserted 2.4 mm deep.
Two 2-mm diameter holes were bored 8 mm rostral to the bregma
and 2 mm lateral to the midline separately.
After sectioning of the rat hippocampus, coronal slices of thickness 5 micron were transferred at a distance of 2.5 to 3 mm posterior to Bregma
on silanated slides.