Lack of CK5 or ADH-5 (the breast marker cocktail) staining (Figure 4, E through H) and uniform ER expression are helpful clues for the diagnosis of IP with ADH/LG-DCIS.
Because of the high sensitivity and specificity of GATA3 for breast origin, GATA3 has been widely used as a breast marker in routine clinical practice.
Biological variation of the breast marker
CA 15-3: implications for necessary standards of performance and the interpretation of results.
(30) This sensitivity of GCDFP-15 as a breast marker is a function of histologic subtype and is generally greatest in lobular carcinoma (particularly those with signet ring cells) as well as tumors showing apocrine features.
Comparative sensitivities and specificities of antibodies to breast markers GCDFP-15, mammaglobin A, and different clones of GATA3: a study of 338 tumors using whole sections.
(68-74) By application of a breast marker
cocktail (CK5, CK14, CK7, CK18, and p63), UDH exhibits a mosaic staining pattern, and ADH and DCIS show clonal proliferation of luminal epithelial cells with negative HMWCK (basal cytokeratin) staining.
Two IHC cocktails are now commercially available: the Breast Triple Stain (Clarient Inc, Aliso Viejo, California), which is composed of p63, CK5, and CK8/18, and the ADH-5 Breast Marker
Cocktail (BioCare Medical, Concord, California), which has CK7 and CK14 in addition to p63, CK5, and CK18 antibodies.
Unless hormonal therapy is indicated (see below) immunohistochemical measurement of conventional breast markers
such as estrogen receptor (ER), progesterone receptor (PgR), and human epidermal growth factor receptor homolog 2 (HER2) is of limited value in DCIS.
The authorisation makes the company the only sector player with a portfolio of FDA-approved image analysis algorithms and digital read applications for the five key immunohistochemistry (IHC) breast markers
The second article focuses on papillary and nonpapillary breast lesions and their differential diagnosis; the diagnostic and prognostic IHC markers for triple-negative breast cancers, such as proline glutamic acid and leucine-rich protein (PELP1), GATA-binding protein 3 (GATA3), and androgen receptor (AR); and an example with aberrant expression of breast markers
in lung adenocarcinoma.