Blunt-end DNA cloning introduces the insertion of blunt-ended DNA or 5'-end phosphorylated PCR product into a linearized blunt-ended vector (Upcroft and Healey, 1987).
A quikchange-like method to realize efficient blunt-ended DNA directional cloning and site-directed mutagenesis simultaneously.
Rapid and efficient method for cloning of blunt-ended DNA fragments.
Blunt-end DNA cloning is another cloning technique which introduces the inserting of blunt-ended DNA or 5'-end-phosphorylated PCR product into a linearized and blunt-ended vector (Upcroft et al., 1987).
To obtain optimal ligation condition for blunt-ended DNA ligation, several studies were done (Liu and Schwartz 1992; Costa and Weiner 1994).