blunt-end

blunt-end

(blunt-end),
Refers to double-stranded DNA in which no unpaired bases are turned at the end of the polynucleotide.
References in periodicals archive ?
Blunt-end DNA cloning is another cloning technique which introduces the inserting of blunt-ended DNA or 5'-end-phosphorylated PCR product into a linearized and blunt-ended vector (Upcroft et al.
Because of these disadvantages, blunt-end cloning should not be preferred for cloning of very large DNA fragments or constructing of comprehensive mutant libraries (An et al.
For blunt-end cloning, pORE-E3 binary vector carried TaNAC69-1 and TtNAM-B2 genes and pAHC25 monocot expression vector were cut with Sma I and Sac Irestriction enzymes.
TurboLigation[TM] Kit enables quick ligation of both blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature.
Each was cloned into pPCR-Script by blunt-end ligation and transformed into XL-10 Gold Ultracompetent cells.
To keep costs in line, the extrusions used a one-dimensional, blunt-end cut instead of the expected machined face.
The sequences were blunt-end ligated into the EcoR V site of pcDNA3.
The coding sequence of the MT-3 gene was obtained from human proximal tubule cell RNA by RT-PCR, blunt-end ligated into the EcoRV site of pcDNA3.
long blunt-end poles as well as a Coast Guard-approved life ring attached to a rope nearby.