US 9mm circular needle, 74cm length (or size needed to obtain gauge) Stitch marker Blunt-end
yarn needle Fiberfill stuffing
Blunt-end DNA cloning is another cloning technique which introduces the inserting of blunt-ended DNA or 5'-end-phosphorylated PCR product into a linearized and blunt-ended vector (Upcroft et al.
Because of these disadvantages, blunt-end cloning should not be preferred for cloning of very large DNA fragments or constructing of comprehensive mutant libraries (An et al.
For blunt-end cloning, pORE-E3 binary vector carried TaNAC69-1 and TtNAM-B2 genes and pAHC25 monocot expression vector were cut with Sma I and Sac Irestriction enzymes.
TurboLigation[TM] Kit enables quick ligation of both blunt-end
or cohesive-end DNA fragments in just 5 minutes at room temperature.
Each was cloned into pPCR-Script by blunt-end
ligation and transformed into XL-10 Gold Ultracompetent cells.
To keep costs in line, the extrusions used a one-dimensional, blunt-end
cut instead of the expected machined face.
The sequences were blunt-end ligated into the EcoR V site of pcDNA3.
The coding sequence of the MT-3 gene was obtained from human proximal tubule cell RNA by RT-PCR, blunt-end ligated into the EcoRV site of pcDNA3.
poles as well as a Coast Guard-approved life ring attached to a rope nearby.