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The transfer of protein, RNA or DNA molecules from a thick acrylamide or agarose gel to a paper like membrane—usually nylon or nitrocellulose—by capilliarity or an electric field, preserving the spatial arrangement
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References in periodicals archive ?
Brazil, Mexico and countries in rest of Latin America together form the market for western blotting in the Latin American region.
Modernists were not the first to notice the cryptographic possibilities of blotting paper.
Assessing intra-assay variability of phosphorylation p70S6 kinase by Western blotting
Despite recent concerns about reliability of the method and its results, western blotting is an indispensable scientific tool used to quantify relative protein levels, with applications spanning from clinical diagnostics to answering fundamental questions in life science.
In addition, the company's protein marker is ideal to monitor efficiency in electrophoresis and transfer of proteins from gel to membrane following Western blotting and to estimate the molecular weight of protein samples.
Western blotting: Electrophoresis was performed as described by and western blot analysis was performed as described by Laemmli (1970).
Sponge the area with the vinegar solution and again carry out the blotting process.
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
The most common method for analysing protein expression levels is western blotting with detection of a single protein target, using horseradish peroxidase--conjugated or alkaline phosphatase--conjugated antibody probes combined with colorimetric or chemiluminescent detection.
Blotting techniques seem thus more sensitive than the presently used ELISA seropositivity threshold for detecting low anti-PA antibody titers.
Protein blotting and detection; methods and protocols.
The animals were sacrificed after 4-6 weeks, the tumors excised and processed in parallel for Western blotting, and formalin-fixed, paraffin-embedded blocks.