blotting


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blotting

/blot·ting/ (blot´ing) soaking up with or transferring to absorbent material.
The transfer of protein, RNA or DNA molecules from a thick acrylamide or agarose gel to a paper like membrane—usually nylon or nitrocellulose—by capilliarity or an electric field, preserving the spatial arrangement

blotting

a technique used for the detection of DNA, RNA or protein. See northern blot, southern blot, western blot. Called also blot analysis.
References in periodicals archive ?
Western blotting consumables including reagents and kits used for testing in different applications
Assessing p70S6 kinase phosphorylation by phospho-flow cytometry is comparable to the Western blotting method
Although a more elegant solution, multiplex fluorescent western blotting requires optimization of blocking reagents, antibody concentrations and incubation times, while users need to be mindful of challenges like antibody cross-reactivity.
Bio-Rad Quantity One software provides predetermined filter and excitation source settings for blotting applications, making both systems easy to use.
To understand whether the effects of SHS on rat hearts are mediated through activating apoptotic pathways, including mitochondria-dependent and Fas death-receptor-dependent signalings, or through suppressing survival pathways, we examined the cardiac levels of signaling proteins and gene expression in these pathways by performing reverse-transcriptase polymerase chain reaction (RT-PCR), Western blotting, or dot blotting.
First measure an envelope, then cut blotting paper to fit when folded in half.
We saw a need to transform Western blotting, and believe the C-DiGit Blot Scanner will greatly impact the scientific community.
ProteinSimple offers high-throughput Western blotting instruments which boosts lab productivity.
Serological differentiation of murine typhus and epidemic typhus using cross-adsorption and western blotting.
All next month, Nurturing Force will donate 20% of all sales of Refreshment For Your Face Rose Aromatic aromatherapy blotting papers to the National Coalition for Women with Heart Disease.
Some of the topics included are DNA isolation, slot-blot quantification, restriction fragment length polymorphism (RFLP)-Southern blotting, PCR [both dot blot and short tandem repeat (STR) analysis], mitochondrial DNA sequencing, and capillary electrophoresis as well as a review of the statistical evaluation of forensic casework results.
The global market for Western blotting products is estimated at one billion dollars.