blotting


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blotting

/blot·ting/ (blot´ing) soaking up with or transferring to absorbent material.
The transfer of protein, RNA or DNA molecules from a thick acrylamide or agarose gel to a paper like membrane—usually nylon or nitrocellulose—by capilliarity or an electric field, preserving the spatial arrangement

blotting

a technique used for the detection of DNA, RNA or protein. See northern blot, southern blot, western blot. Called also blot analysis.
References in periodicals archive ?
Established as a key laboratory technique for over 30 years, Western blotting requires a complex combination of analytical knowledge, extensive manual handling, dexterity and strict adherence to protocols in order to achieve reliable results.
L03012) specifically designed for high protein blotting efficiency.
As a fast and inexpensive alternative to a live demonstration, I provide an animated slideshow on our school website that has worked well for me as I have walked students through Southern blotting (http://biology.
Sponge the area with the vinegar solution and again carry out the blotting process.
To understand whether the effects of SHS on rat hearts are mediated through activating apoptotic pathways, including mitochondria-dependent and Fas death-receptor-dependent signalings, or through suppressing survival pathways, we examined the cardiac levels of signaling proteins and gene expression in these pathways by performing reverse-transcriptase polymerase chain reaction (RT-PCR), Western blotting, or dot blotting.
Practice blotting the painted leaf on scrap paper, then blot it onto the torn shape.
PAVEMENT: Crabtree & Evelyn Facial Blotting Tissue, pounds 2.
In the method of the present paper, a standard RPA was performed, including probe hybridization and RNase treatment, and then the remaining RPA products were bound to a membrane via slot blotting.
A performance took place, but there was no carved flesh, no gore, no HIV-infected blotting paper dangled overhead, no audience flight from the theater in a health-hazard panic.
Just choose a motif, draw it on graph paper, poke holes through the graph paper into the blotting paper, and fill in with stitches (we used Algerian eye stitches, backstitches, and running stitches).
In this procedure, which costs about $65, suspect blood is added to blotting paper that contains AIDS virus proteins of different sizes.