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North·ern blot a·nal·y·sis

a procedure similar to the Southern blot analysis, used to separate and identify RNA fragments; typically through transferring (blotting) RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]

blot

(blŏt)
n.
The Northern, Southern, or Western blot analyses.

blot

noun A nitrocellulose or nylon membrane bearing a biomolecule of interest—e.g., DNA, RNA or protein—transferred to the membrane from an electrophoretic gel by either osmosis or vacuum; after transferring the molecule of interest, the membrane is bathed in a solution containing a “mirror-image” of the molecule already on the membrane, producing a hybridisation.
 
verb To perform any of a number of similar techniques used to analyse biologic molecules. Mixtures of biomolecule—DNA, RNA or protein—fragments are separated by electrophoresis through a polymeric (agarose or polyacrylamide) gel; the separated components are then transferred to a membrane where they are immobilised, analysed and visualised by various techniques including antibody binding and DNA hybridisation.

Southern blot
A technique for identifying the presence or absence of a segment of DNA in a sample; the procedure begins by partial enzymatic digestion of nucleic acids, cutting the DNA at specific sites by any of a number of restriction endonucleases, each of which recognises and cuts at a 5- or 6-nucleotide sequence. For instance, HindIII, from H influenzae, cuts DNA at all sites bearing the nucleotide sequence A/AGCTT at the mirror image sites on the 2 DNA chains between the 2 adenines A/A, resulting in a mixture of DNA fragments of varying lengths measuring up to 30 kD, which is then electrophoresed in an agarose gel and transferred to a membrane. Because the DNA on the membrane is single stranded, it has a high affinity for its complementary strand. The final step in the blot is to radioactively “tag” the complementary DNA (Southern blot, after Dr E. Southern), RNA (Northern blot), protein (Western blot), DNA-protein hybridisation (Southwestern blot), and RNA-protein hybridisation (Northwestern blot).

Dot blot
DNA, RNA, or protein are dotted directly onto a membrane support, and form discrete spots.

North·ern blot a·nal·y·sis

(nōr'dhĕrn blot ă-nal'i-sis)
A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically done through transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]

South·ern blot a·nal·y·sis

(sŭdh'ĕrn blot ă-nal'i-sis)
A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridized with complementary (labeled) nucleic acid probes.

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]

zoo blot a·nal·y·sis

(zū blot ă-nal'i-sis)
A procedure using Southern blot analysis to test the ability of a nucleic acid probe from one species to hybridize with the DNA fragment of another species.
References in periodicals archive ?
A highly defective HIV-1 strain isolated from a healthy Gabonese individual presenting an atypical Western blot. AIDS.
* Hybridizing the patient DNA on the blot with the labeled probe
Our present results show that CVs of 20% for q-PCR and 37% for Southern blot could not be avoided in age-matched controls, regardless of the tissue studied.
Using the A4136G substitution associated with Leber hereditary optic neuropathy as a prototype, we have validated a technical approach to the preparation of synthetic positive controls suitable for ASO dot blot analysis in any disease.
The same blot was subsequently stripped at 50[degrees]C for 30 min in a buffer containing 62.5 mM Tris (pH 6.7), 2% SDS, and 100 mM 2-mercaptoethanol.
Summary: New Delhi [India], Dec 30 (ANI): The Delhi unit of Narcotics Control Bureau (NCB) on Saturday arrested four college-going students and seized 1.140 kg of Cannabis as well as three LSD blot papers.
Penning a flirtatious letter to typist Martha Clifford, he quickly blots the address to hide his infidelity from an onlooker.
Studies on HKPs have shown that total protein staining of the gels or blots is better suited than HKPs to correct for differences in loading.
Although Southern and Northern blots illustrate important principles about nucleic acid chemistry and hybridization, they are very difficult to describe in lecture: it is an elaborate process to separate nucleic acid molecules by size, move the nucleic acid from gel to blot, radioactively label a probe, hybridize the probe to a target sequence on the blot, and detect the hybridized probe by exposure of the blot to x-ray film.
The borrelial antibodies profile obtained in the blots for the studied population sample and in the individual blots estimated as positives were analyzed and described.
This new platform combines highly-sensitive quantitative detection of multicolor fluorescent Western blots with key ease-of-use features aimed at simplifying the laboratory imaging process.