blood smear


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blood smear

a blood test used to provide information concerning drugs and diseases that affect the morphology of red and white blood cells and to help diagnose certain congenital and acquired diseases. A properly performed and analyzed blood smear is the most informative of all hematologic tests, allowing examination of erythrocytes, platelets, and leukocytes.
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PREPARATION OF BLOOD SMEAR
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PREPARATION OF BLOOD SMEAR

blood smear

A drop of (anticoagulated) whole blood spread thinly on a glass microscope slide so that blood cell types can be examined, counted, and characterized. Synonym: peripheral blood smear

Procedure: The slide must be grease-free. It is cleaned with alcohol, rinsed in warm water, and wiped clean with a lint-free towel or lens paper.A small drop of blood is placed on the slide; the end of another slide (spreader slide) is placed against the first slide at a 45° angle and pulled back against the drop of blood so that the drop spreads between the point of contact of the two slides. Then the spreader slide is pushed forward against the first slide; the blood will form an even, thin smear. The slide is dried by waving it in the air; it should not be heated. The blood smear is covered with Wright's stain and allowed to stand 2 min. An equal amount of distilled water or buffer solution is added and mixed uniformly. It is allowed to stand 5 min. The stain is gently washed off and the slide is allowed to dry.

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See also: smear
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This report describes a case of recurrence of TTP in the absence of schistocytes in the peripheral blood smear during the recurrent episode.
Repeat blood smears prepared 6 h post imidocarb dipropionate administration were still positive for B.
ovale on blood smear (percent parasitemia unknown) and by PCR.
On average, 220 blood smears and 170 body fluid smears are reviewed monthly, which provides a substantial test volume on which to evaluate the clinical usefulness of the test.
Based on clinical symptoms, blood smear examination and histopathology, outbreak was confirmed as haemoproteus infection in white fantail pigeons.
Traditionally, morphological examination of the peripheral blood smear has been performed by manual use of a microscope.
The pipeline is designed to run on large series of images, such as in our study, where it was run on 104 images per blood smear.
During testing for thrombocytopenia, a peripheral blood smear was obtained on hospital day 1 and showed ring forms with tetrads (Figure) and a parasitemia level of 10%.
Although automatic blood analyzers are widely used, peripheral blood smear is the hematological gold standard for definitive diagnosis.
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The results of rapid diagnostic test for malaria (OptiMAL test, Diamed AG, Switzerland) were correlated with the peripheral blood smear findings in the patients in whom it was requested.