Q When we get a negative result on a rapid strep Group A, we set up a blood plate
For example, by applying "if and then" rules, the system could flag if the patient is a pregnant woman or is of child-bearing age, then look for small numbers of white Group B Strep colonies on the chromogenic plates or look for small numbers of hemolytic colonies on blood plates.
A recent study has been published on software that can "read digital images and provide quantitation of Colony Forming Units from blood agar plates." (4) The software counts Colony Forming Units (CFUs) on blood plates, and segregates no-growth cultures and can categorize growth--for example 0-10 CFUs, 10-100 CFUs, and >100 CFUs--which helps overall "to reduce the cost of urine cultures by sorting plates based on colony growth." (4) The study concluded that the quantitative software was accurate at discerning no-growth without any false results.
The young girl's health condition deteriorated, when she developed a brain haemorrhage after a blood plates
transfusion was not carried on her in time.
For the isolation of anaerobes, specimens were plated onto pre-reduced vitamin Kl enriched Brucella blood agar (consisting of 5% sheep blood), anaerobic blood agar plates containing kanamycin and vancomycin, and anaerobic blood plates
containing colistin and nalidixic acid, and then inoculated into enriched thioglycolate broth.
Sterile 6 mm diameter filter paper discs saturated with fentanyl solution were applied to Columbia agar with 5% horse blood plates
containing 107 colony forming units.[ml.sup.-1] of reference bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 51299).