(29) After incubation, follicles were evaluated with a stereoscopic microscope to determine fertilization by identifying the presence of blastomeres
This seemed to occur simultaneously in both blastomeres
Polar Body, Blastomere
and Trophectoderm (TE) Cells Biopsy
Isolated genomic DNA from control adults (Genomic) and WGA from a blastomere
were used as templates for GAPDH, SRY, and DYS14 qPCR reactions.
Approximately 1 [micro]g MDA product from each blastomere
was used for library construction and sequencing.
The shape of the blastomeres
was round, such that it was reasonable to perform circle fitting for each blastomere
Fluorescence in situ hybridization analysis of two blastomeres
from day 3 frozen-thawed embryos followed by analysis of the remaining embryo on day 5.
Hamilton Thome's LYKOS laser system is used for intricate clinical procedures such as laser-assisted hatching, trophectoderm biopsy and blastomere
(32) The biopsy is performed using one of three methods: extraction of the polar bodies from the pre-embryo oocytes; (33) a one to two cell extraction at the day three blastomere
stage; (34) or a one to two cell extraction at the day five blastocysts stage.
biopsies were performed from each embryo to reduce the number of embryos needed for the research.
(2000) found that nearly 45% of thawed embryos suffered some amount of blastomere
(cell) loss, causing an approximate 30% reduction in implantation.
The problem of abnormally large offspring was first described for calves born from blastomere
NT procedures (6, 7) but was subsequently shown to occur in embryos produced by IVF (8, 9) and SCNT (10, 11, 12, 13) procedures.