In addition, further analysis of microtubule and microfilament arrays during ooplasm segregation and in the presence of cytoskeletal inhibitors will further extend our understanding of the mechanism of blastodisc
formation and early development in the squid embryo.
Eggs treated with 1% DMSO formed a blastodisc (volume at [T.
1993a): (1) oil droplets did not segregate to the vegetal pole but instead floated to the portion of the egg that was uppermost during the experiment - equator [ILLUSTRATION FOR FIGURE 1C OMITTED], animal pole [ILLUSTRATION FOR FIGURE 2C AND D OMITTED], or vegetal pole; (2) a smaller-than-normal blastodisc formed [ILLUSTRATION FOR FIGURE 1C OMITTED]; (3) saltatory motion was absent; and (4) the eggs did not cleave.
2]) inhibited both the growth of the blastodisc and the movements of oil droplets.
In all these eggs, the droplets of injected fluids moved toward the animal pole and came to rest in the blastodisc [ILLUSTRATION FOR FIGURE 6B-D OMITTED].
However, in one egg in which the injected droplet was not observed to fuse with endogenous droplets, the injected droplet moved up, toward the animal pole, and came to rest near the blastodisc, while nearby endogenous droplets moved by it in the opposite direction on their way to the vegetal pole.
The inhibition of cytoplasmic streaming and formation of the blastodisc by CCD in medaka eggs is consistent with data obtained from other teleost eggs (Katow, 1983; Ivanenkov et al.
2+] inhibits formation of the blastodisc (Fluck et al.
Though the growth of the medaka blastodisc is slowed by microtubule poisons (Abraham et al.