biuret


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Related to biuret: Biuret test, Biuret reagent

biuret

 [bi´u-ret]
a urea derivative; its presence is detected after addition of sodium hydroxide and copper sulfate solutions by a pinkish-violet color (protein test) or a pink and finally a bluish color (urea test).
biuret reaction the reaction in biuret tests.
biuret test either of the tests done with biuret.

bi·u·ret

(bī-yū-ret'),
A derivative of urea obtained by heating, eliminating one NH3 between two ureas. Used in protein determinations.
Synonym(s): carbamoylurea

biuret

A white crystalline substance, which is intermediate between urea and cyanuric acid, and formed by heating urea.

bi·u·ret

(bī'yŭr-et')
Obtained by eliminating one NH3 between two urea molecules. Used in protein determinations.
Synonym(s): carbamoylurea.
References in periodicals archive ?
Balakhontsev EN, Iskhakov FM, Pakhomova LM, Bokarev KS (1981) Effect of foliar application of biuret on sugar beet productivity.
In continuation of studies on ureic systems (urea, its derivatives and analogs) we report results of quantum mechanical study on molecular structure and stability of biuret and its sulfur analogs; [H.sub.2]NCXNHCXN[H.sub.2], X = O, S.
Biuret method was used for the estimation of protein contents of SK enzyme (Gornall et al., 1949).
Samples (15 [micro]g protein, determined by the biuret method) were loaded onto a polyacrylamide gel made of 10% separating gel and 4% stacking gel and subjected to electrophoresis at a constant current of 20 mA/gel, using an electrophoresis apparatus (AE-6440, Atto Co., Tokyo, Japan).
But, biuret and cyanuric acid which may cause deposit are sometimes generated by the chemical reactions shown in equation 3, 4 and 5 without generating N[H.sub.3] [3].
Modified biuret method was used to determine T.S.P [20], while albumin concentration was measured using a method based on albumin ability of binding bromocresol green (BCG) [21].
The presence of proteins was also confirmed by different biochemical tests including Biuret Test [23], lead acetate test [23, 25], Copper Complex Test [26] and Combur Test [11].
Blood was sent for routine testing; serum total protein was 13.6 g/dL by the biuret method (reference interval, 6.1-7.9 g/dL).
Serum Total protien (10-13) estimation was done by Biuret method: The peptide bonds of protein react with the copper II ions in alkaline solution to form blue -violet complex (biuret reaction).
Serum level of total protein was measured by Biuret's method.
Total protein determination: This was determined by the biuret method (Gornall et al.
Serum total protein was determined using the biuret method; cupric ions in the biuret reagent react with the peptide bonds of the protein molecule in an alkaline solution to form a blue colored complex [14].