Balakhontsev EN, Iskhakov FM, Pakhomova LM, Bokarev KS (1981) Effect of foliar application of biuret
on sugar beet productivity.
In continuation of studies on ureic systems (urea, its derivatives and analogs) we report results of quantum mechanical study on molecular structure and stability of biuret
and its sulfur analogs; [H.sub.2]NCXNHCXN[H.sub.2], X = O, S.
method was used for the estimation of protein contents of SK enzyme (Gornall et al., 1949).
Samples (15 [micro]g protein, determined by the biuret
method) were loaded onto a polyacrylamide gel made of 10% separating gel and 4% stacking gel and subjected to electrophoresis at a constant current of 20 mA/gel, using an electrophoresis apparatus (AE-6440, Atto Co., Tokyo, Japan).
and cyanuric acid which may cause deposit are sometimes generated by the chemical reactions shown in equation 3, 4 and 5 without generating N[H.sub.3] .
method was used to determine T.S.P , while albumin concentration was measured using a method based on albumin ability of binding bromocresol green (BCG) .
The presence of proteins was also confirmed by different biochemical tests including Biuret
Test , lead acetate test [23, 25], Copper Complex Test  and Combur Test .
Blood was sent for routine testing; serum total protein was 13.6 g/dL by the biuret
method (reference interval, 6.1-7.9 g/dL).
Serum Total protien (10-13) estimation was done by Biuret
method: The peptide bonds of protein react with the copper II ions in alkaline solution to form blue -violet complex (biuret
Serum level of total protein was measured by Biuret
Total protein determination: This was determined by the biuret
method (Gornall et al.
Serum total protein was determined using the biuret
method; cupric ions in the biuret
reagent react with the peptide bonds of the protein molecule in an alkaline solution to form a blue colored complex .