Mice transplanted with murine bone marrow (BM) cells expressing CALM/AF10 fusion transcripts developed an aggressive form of biphenotypic
(34) Hypotheses include a coincidence, (3,6,10,24,27,34,36) exposure, such as to a virus or carcinogen, (3,10,20,23,24,27,28,34,36) genetic predisposition to lymphoma development, (27,34,36) interactions between lymphoma cells and surrounding lymphocytes, (10,17,24,27,34,36) immunodeficiency or immune dysregulation, (3,16,17,24,27,28,34) or as biphenotypic
manifestations of the same neoplastic clone.
Fuliminant septicemia of Bacillus cereus resistant to carbapenem in a patient with biphenotypic
A recent study described a novel technique for the identification and classification of CTCs into three subpopulations based on the expression of epithelial (E-CTC), biphenotypic
epithelial/mesenchymal (E/M-CTC), and mesenchymal (M-CTC) markers .
Some tumor cell nests appeared to be comprised entirely of cells with a basaloid phenotype and a suggestion of peripheral palisading, while other nests appeared somewhat morphologically biphenotypic
with slightly smaller and darker peripheral myoepithelial-like cells as well as a second population of central cells with more abundant and eosinophilic cytoplasm and a tendency toward spindling or whorling (Figure 3).
Among MPAL, 19(83%) cases were Biphenotypic
[13(57%) cases of My/B-ALL, 5(22%) cases of My/T-ALL, and 1(4%) case of T/B-ALL].
Furthermore, acute lymphatic leukemia sub segmented into acute biphenotypic
leukemia, burkitt leukemia, precursor B acute lymphatic leukemia, and precursor T acute lymphatic leukemia.
Leukaemias under this category were given many different names, including bilineage leukaemias, biphenotypic
leukaemias, hybrid leukaemias, undifferentiated leukaemias.
Number of Sex Age Disease Degree of GVHD patient (years) 01 F 49 AML M2 I 02 F 32 NHL IIIB I 03 F 19 MDS IV 04 M 55 MDS III 05 M 56 MDS CMV 06 M 44 AA 0 07 M 35 AA I 08 M 18 NHL HSV 09 F 26 AA I 10 M 39 AML M5 III 11 M 28 ALL L2 III 12 F 18 AML M5 IV 13 F 32 AA 0 14 F 37 AA Multiple bacterial infections 15 M 44 AML M2 III 16 F 46 Biphenotypic
leukemia 0 17 M 29 AA 0 18 M 44 AA III AA: aplastic anemia; AML: acute myeloid leukemia; MDS: hypoplastic myelodysplastic syndrome; NHL: non-Hodgkin lymphoma; ALL: acute lymphoblastic leukemia; GVHD: graft-versus-host disease; HSV: herpes simplex virus; CMV: cytomegalovirus.
Generally, acute leukemias can be classified as either lymphoid or myeloid based on the lineage of the blastic cells, but rarely blasts of both lineages can be present (so-called bilineage acute leukemias) or blasts can express antigens of more than 1 lineage (so-called biphenotypic
For T-lineage ALL, the panel consisted of one tube as follows: CD8 v450, CD2 FITC, CD5 PE, CD34 PE-TR, CD56 PE-Cy5, CD3 PE-Cy7, CD4 A594, CD7 APC, CD30 APC-A700, and CD45 APC-H7 For biphenotypic
leukemia (two patients), the same combination of reagents used for B or T cell ALL was used and supplemented by a combination directed at abnormal myeloid progenitors ((1) HLA-DR PB, CD15 FITC, CD33 PE, CD19 PE-TR, CD117 PE-Cy5, CD13 PE-Cy7, CD38 A594, CD34 APC, CD71 APC-A700, CD45 APC-Cy7, (2) HLA-DR PB, CD64 FITC, CD123 PE, CD4 PE-TR, CD14 PE-Cy5.5, CD13 PE-Cy7, CD38 A594, CD34 APC, CD16 APC-A700, CD45 APC-Cy7, and (3) CD56 A488, CD7 PE, CD5 PE-Cy5, CD33 PE-Cy7, CD38 A594, CD34 APC, CD45 APC-Cy7).
Borrello et al., "Biphenotypic
B/macrophage cells express COX-1 and up-regulate COX-2 expression and prostaglandin E(2) production in response to pro-inflammatory signals," European Journal of Immunology, vol.