biotinylation


Also found in: Wikipedia.

biotinylation

The covalent linking of biotin to a protein, peptide, nucleic acid (DNA, RNA) or other biomolecule of interest, which in turn binds with high affinity to avidin or streptavidin linked to an enzymatic moiety or fluorescent molecule. Biotinylation is used in detector systems and to isolate biotinylated biomolecules of interest.
References in periodicals archive ?
As the range of biological targets increases, so the demand for synthesis of radiolabeled versions expands to include carbon-14 peptides, modified carbohydrates, polysaccharides and glycoconjugates, in addition to PEGylated materials, BIOTINylation and biopolymers.
The significance of biotinylation was confirmed by the facilitated absorption of BLPs through receptor-mediated endocytosis as well as by the improved physical stability of the liposomes.
During the incubation period, the anti-histone antibody binds to the histone component of the nucleosomes and simultaneously captures the immunocomplex to the streptavidin-coated microplate via its biotinylation.
Highly efficient synthesis in this variation of hybrid capture is coupled with biotinylation only of full-length product, thereby ensuring only the complete oligos are enriched, improving sequencing reliability.
The blood brain barrier is formed by claudins, and claudin-5 knockout in mice changes the permeability of the blood brain barrier even to a primary amine-reactive biotinylation reagent.
Blood samples were analyzed using a Coulter Counter and red blood cell (RBC) survival was measured by biotinylation.
1) Develop a novel tool to enrich early stage gametocytes from parasite culture using cell-type specific surface biotinylation and affinity purification.
Up to now, Creative Biomart has nearly six kinds of protein labeling service: stable Isotope Labeling, Protein Biotinylation, Fluorescent Labeling in vitro, Fluorescent Tagged Protein in vitro, Enzyme/Antibody conjugation and Site-specific labeling, the newly launched one.
For labeling and biotinylation of the genomic DNA, a site-specific labeling approach was used as published by Monecke and Ehricht [17].
Subcellular localization of transporters along the rat bloodbrain barrier and blood-cerebral-spinal fluid barrier by in vivo biotinylation.
In vivo biotinylation demonstrates that reticulated platelets are the youngest platelets in circulation.