biotinylation


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biotinylation

The covalent linking of biotin to a protein, peptide, nucleic acid (DNA, RNA) or other biomolecule of interest, which in turn binds with high affinity to avidin or streptavidin linked to an enzymatic moiety or fluorescent molecule. Biotinylation is used in detector systems and to isolate biotinylated biomolecules of interest.
References in periodicals archive ?
M2 PRESSWIRE-September 4, 2019-: Profacgen Enhanced Its Protein Biotinylation Service Recently
The biotinylation procedure of the antibodies is presented in the online Data Supplement.
The metabolic interactome of CA IX determined using the proximity-dependent biotinylation proteomic approach, recently, revealed a novel role of this isoform in tumor migration and invasion [138].
Optimization of reagent's amount for biotinylation is the key step to overcome this aggregation issue [139].
Lo, "Design of luminescent biotinylation reagents derived from cyclometalated iridium(III) and rhodium(III) bis(pyridylbenzaldehyde) complexes," Inorganic Chemistry, vol.
The latter include histone acetylation, methylation, phosphorylation, ubiquitination, summation, biotinylation, and citrullination.
Biotinylation of one of the primers for PCR was carried out with the aim of pyrosequencing the amplicon.
The biotinylation experiments and functional assays will be done to further investigate the transportation of flats [52].
In this study, we used immunohistochemistry, Western blotting and surface biotinylation to evaluate the intracellular and cell membrane expression of RyR2 protein following transfection of Human Embryonic Kidney (HEK) cells with such cDNA RyR2.
As the range of biological targets increases, so the demand for synthesis of radiolabeled versions expands to include carbon-14 peptides, modified carbohydrates, polysaccharides and glycoconjugates, in addition to PEGylated materials, BIOTINylation and biopolymers.
The significance of biotinylation was confirmed by the facilitated absorption of BLPs through receptor-mediated endocytosis as well as by the improved physical stability of the liposomes.
During the incubation period, the anti-histone antibody binds to the histone component of the nucleosomes and simultaneously captures the immunocomplex to the streptavidin-coated microplate via its biotinylation. At the same time, the anti-DNA-POD antibody reacts with the DNA component of the nucleosomes.