Methanol washes of blue tips were subjected to positive ion-mode electrospray mass spectrometry (Waters Mariner
BioSpectrometry Workstation oa-TOF); these analyses confirmed the presence of NP compounds, marketed as surfactants from the Tergitol NP series.
All laser desorption/ionization-mass spectrometry data was acquired with an Applied Biosystems Voyager-DE
Biospectrometry Workstation (Foster City, California) benchtop matrix-assisted laser desorption/ionization time-of-flight mass spectrometer.
0 Windows NT-based software for its Voyager-DE
Biospectrometry Workstations, mass spectrometry instruments that employ MALDI-TOF for the characterization of peptides, proteins and DNA in research and drug discovery.
The Mariner
Biospectrometry Workstation combines the versatility of electrospray ionization with powerful time-of-flight mass analysis to generate mass spectra with high mass accuracy and resolution, all in a benchtop instrument.
MALDI-TOF MS experiments were performed with a Voyager Elite
biospectrometry research station with delayed ion extraction (PerSeptive Biosystems, Framingham, Massachusetts).
According to Joe Malandrakis, vice president and general manager of PerSeptive Biosystems, "This agreement allows Sequenom to supply their customers with the Voyager-DE
Biospectrometry Workstations to obtain the accurate results required for successful mass analysis.
Reflectron MS analyses were performed on a Voyager DE PRO
Biospectrometry Workstation (Applied Biosystems) equipped with a pulsed laser beam (nitrogen laser, [lambda] = 337 run).
The Proteomics Solution 1 System includes the SymBiot I Sample Preparation Workstation and the Voyager-DE PRO
Biospectrometry Workstation, a MALDI-TOF benchtop spectrometer.
MALDI-TOF spectra (600-4000 Da) were acquired in the positive reflectron mode with a Voyager DE-STR
Biospectrometry Workstation mass spectrometer (Applied Biosystems) with an accelerating voltage of 20 kV (delayed extraction time, 200 ns; ~250 scans averaged for each acquisition).
With the work that our scientists have completed, we can now look to a new set of enabling tools based upon Delayed Extraction MALDI-TOF/MS that will revolutionize the speed and precision with which researchers in the life sciences industry can sequence and understand that structure of complex peptides, proteins and oligonucleotides using
biospectrometry.
