bioluminescence imaging


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bioluminescence imaging

A noninvasive imaging modality which is widely used in pre-clinical oncology research in a range of activities—e.g., imaging animal tumour models by luciferase-expressing cells after administering a substrate, drug development, gene monitoring, evaluating tumour development, metastasis and protein interactions.
References in periodicals archive ?
Antrodia camphorata suppresses lipopolysaccharide-induced nuclear factor-[kappa]B activation in transgenic mice evaluated by bioluminescence imaging.
It's not just about anatomy: in vivo bioluminescence imaging as an eyepiece into biology.
In the published research, Caltech and Children's Hospital Los Angeles investigators created a mouse model of Ewing's sarcoma that mimics the tumor localizations in humans and also provides for simultaneous, real-time bioluminescence imaging of the disseminated tumors by using human Ewing's sarcoma cells engineered to express luciferase.
Current commercial and academic efforts have produced a variety of luciferases that have been engineered and codon-optimized from different species for bioluminescence imaging in live samples (Thorne et al.
Bioluminescence imaging typically requires long exposure times, which limit spatiotemporal resolution: in some applications, it can require specialized equipment (Bell et al.
A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo.
By isolating the pancreas and using bioluminescence imaging (which relies on a protein found in the firefly), the researchers determined that a circadian clock is expressed autonomously in the pancreas.
Examples of topics discussed include validation of bioluminescent imaging techniques, imaging vasculature and lymphatic flow in mice using quantum dots, bioluminescent imaging of transplanted islets, detection of apoptosis using cyclic luciferase in living mammals, noninvasive bioluminescent imaging of infections, real-time bioluminescence imaging of viral pathogenesis, bioluminescent monitoring of in vivo colonization and clearance dynamics by light-emitting bacteria, and novel tools for use in bioluminescence resonance energy transfer.
Researchers at Caltech and CHLA created a mouse model of Ewing's sarcoma that mimics the tumor localizations in humans and also provides for simultaneous, real time bioluminescence imaging of the disseminated tumors by using human Ewing's sarcoma cells engineered to express luciferase.
Various imaging technologies, including magnetic resonance imaging (MRI), bioluminescence imaging and photoacoustic imaging can be used for monitoring cellular biodistribution and assessing the effect of the cells on host organs and tissues, but no single technique provides high sensitivity, spatial and temporal resolution, and functional and anatomical imaging.
In the technique, dubbed "cell-specific in vitro bioluminescence imaging," or CS-BLI, the cancer cells in each sample are equipped with a gene that makes them glow - a process unaffected by the normal cells nearby.