Agrobacterium-mediated transformation (AMT) is the method of choice for sorghum transformation as it is a simple, low cost and highly efficient alternative to direct gene delivery methods such as biolistics.
The majority of reports where transformation is successful have relied upon a biolistic approach, even though Agrobacterium-mediated transformation has been accomplished recently .
Therefore, the success of any cotton improvement programme using gene transfer technological tools such as biolistics
and Agrobacterium-mediated transformation depends on the availability of plant regeneration systems that are genotype-independent, efficient and which do not yield somaclonal variant (Firoozabady et al.
Particle bombardment technology, also known as biological biolistics or bioballistics, is a nonviral, physical gene delivery method in which target cells are transfected using DNA-coated particles.
Bio-Rad Laboratories is the leading vendor of systems and consumables for biolistic gene delivery.
This technology -- combined with Ball's exclusive rights to the Biolistics
"gene gun" transformation technology in floricultural plants -- will allow Ball and Agritope to develop novel flower products that have direct consumer and grower benefits.
and his colleagues are also growing new-fangled cassava, but they employ a different technique, biolistics.
In more recent experiments, however, they have also inserted genes to make the plant resistant to the cassava common mosaic virus, found in South America, and to the much more destructive African cassava mosaic virus, says Claude Fauquet of Scripps, a coauthor of the biolistics report.
And, by virtue of its early filing date, the patent clearly demonstrates Pioneer's early entry into corn transformation with biolistics.
Pioneer filed its application for the patent in June, 1988 -- more than 18 months before others applied for patents in biolistic plant transformation.
is a specific gene delivery method wherein DNA-coated particles are mechanically accelerated into cells.