After TLC separation, the antimicrobial activity of the essential oils was determined by direct bioautography
. (18,19) Pseudomonas aeruginosa ATCC 13388, Staphylococcus aureus ATCC BAA 1026, Candida albicans ATCC 24433, and Escherichia coli NRRL B-3008 strains were used for bioautography
on the TLC plates showed that all the evaluated extracts had antioxidant compounds, which was evidenced by the white bands revealed with DPPH solution.
separates the compounds from a complex mixture through thin-layer chromatography (TLC) and determines the antimicrobial activity of the isolated substances.
Morlock, "Bioprofiling of unknown antibiotics in herbal extracts: Development of a streamlined direct bioautography
using Bacillus subtilis linked to mass spectrometry," Journal of Chromatography A, vol.
In a manner that might also be beckoned in the word autobiography, bioautography
emphasizes the syllable graph as pointing to the plural potentialities of signage.
assay has shown that the whole plant extract exhibits antioxidant compounds, and their intensity is shown by the multitude of the yellow spots on the TLC for a particular extract.
HPTLC Screening for Inhibitors of AChE and BuChE: Direct Bioautography
Detection of antimicrobial compounds by bioautography
of different extracts of leaves of selected South African trees species, Afr.
Antibacterial activities of Dodonaea viscosa using contact Bioautography
is the latest technique employing combinatorial chemistry and high throughput screening used for preliminary screening of biological activities like antimicrobial antioxidant and enzyme inhibition of natural products (Cheng and Wu 2013).
In addition, the antioxidant compounds can be detected using bioautography
method which is quick, low-cost, and allows a better bioassay directed fractionation of bioactive compounds .
Active crude extracts are chosen for column chromatography due to its relatively low complexity as seen with TLC, bioautography
, and disc diffusion method .