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The frequency of micronucleus lymphocytes with P at a concentration of 2.5 [micro]g/mL was insignificant as compared to control samples, while the combination of CUR (25 [micro]g/mL) and P (2.5 [micro]g/mL) increased significantly the frequency of micronuclei in binucleated lymphocytes as compared to control samples (Table 1, Figure 2).
Caption: Figure 3--Mean of binucleated micronucleus lymphocytes exposed for 24 hours to the following: C + Doxorubicin (0.02 [micro]g / mL culture medium); C- (culture medium); P-25 (C.
The frequency of micronuclei induced in vitro by 1.50 Gy X-ray radiation in cultured blood lymphocytes from human volunteers examined treated at different doses of febuxostat Group % micronuclei in binucleated human lymphocyte Sample I (#) II III Mean[+ or -]SD Control 0.45 0.25 0.54 0.41[+ or -]0.15 IR 7.67 7.54 7.68 7.63[+ or -]0.08 (a) FB 1+IR 6.73 6.86 6.69 6.76[+ or -]0.09 (b) FB 10+IR 6.50 6.68 5.99 6.39[+ or -]0.36 (b) FB 50+IR 5.38 5.47 5.27 5.37[+ or -]0.09 (b,c) FB 100+IR 4.57 4.26 4.35 4.39[+ or -]0.16 (b,c) FB 1 0.49 0.59 0.58 0.55[+ or -]0.05 FB 10 0.74 0.87 0.76 0.79[+ or -]0.06 FB 50 0.89 0.85 0.93 0.89[+ or -]0.04 (d) FB 100 0.94 1.03 1.02 0.99[+ or -]0.05 (d) (#) I, II, and III are the number of volunteers who participated in the study, and whole blood was obtained here.
Therefore, they are extensively acknowledged as an endpoint for the estimation of DNA damage.5 Binucleated (BN) cell is most commonly present in cancer cells and may appear as a result of different type of causes; the increased rate of binucleation is usually not preferably a definite indicative parameter.
Presence of MN with binucleated cells (cell with two nuclei) is commonly found in the cancer cells.
One thousand binucleated (BN) cells with two macronuclei surrounded by cytoplasm were scored from each patient and control subject.
The cells overexpressing 17a RNA also revealed a high frequency of several other phenotypic events including binucleated (13.8%) and polylobed nuclei (23.2%) and "stars" (39.7%).
Binucleated and multinucleated cells as well as karyomegaly were multifocally observed.
To validate the results obtained by in Vitro Microflow kit, the analysis of MN in binucleated cells was performed by adding cytochalasin B (Sigma-Aldrich; final concentration of 6 [micro]g/mL) after 44 h of culture .
Monitored values included number of mononucleated, binucleated, and multinucleated cells, incidence of micronuclei, and nuclear division index (NDI).
The mean MN frequency in control PBLs was 10 MN per 1000 binucleated cells.
Some hepatocytes are binucleated. In the liver bars, the surface of each hepatocytes contacts the blood pocket wall, while the other surface contacts the adjacent cells and have in between Bile canaliculus.
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