The target antigen will bind and interact with the TAC antigen binding domain
, expressed at high levels by most of tumor cells to trigger sufficient immune response.
ALX148, designed for combination therapy, is a fusion protein comprised of an engineered high affinity CD47 binding domain
It is a transcription factor and its three domains are firstly the DNA binding domain
, secondly the ligand binding domain
and thirdly the hinge region connecting the two.
The gene is translated into a protein known with three functional domains, including the N-terminal domain (NTD), the DNA binding domain
(DBD) and the C-terminal ligand binding domain
It has been observed that the kinase domain of a CDPK may be active through interaction of C-terminally located calcium binding domain
(Zhanget al., 2010).
Eukaryotic TFs are generally thought to be composed of multiple functional domains: a DNA binding domain
that recognizes a specific DNA sequence, a transcriptional regulation domain, and allosteric regulation domain(s) [2, 3].
Abbreviations: signal peptide: SP; receptor- binding domain
(54-201): RBD; glycan cap (201-309): GC; mucin-like domain (305-485): MLD; internal fusion loop (524-539): IFL; heptad repeats (554-595, 615-634): HR; transmembrane region (651-671): TM.
To create this novel hydrogel, the team assembled genetically-engineered proteins into molecular networks by stitching together the photoreceptor C-terminal adenosylcobalamin binding domain
(CarHC) proteins at room temperature.
Oryza sativa cDNA clone J065152E11 with assigned accession number AK241364.1 was acquired from NIAS Databank of Japan and primers were designed specific for full length and DNA binding domain
containing region of OsDOF18 containing BamHI and XhoI sites to aid in the cloning (Supplementary Table 1).
In case of ZFNs, while examples abound with respect to the binding efficiency of canonical C2H2 binding domain
containing ZFNs, investigations on the utility of noncanonical ZFNs such as those containing C3H1 binding domain
have demonstrated high levels of binding efficiency .
The structure of PPARs has been studied extensively using X-ray crystallography, molecular modeling, and solvent mapping techniques with respect to the DNA binding domain/region (DBD), ligand binding domain
(LBD), and coactivator binding site, providing insight into the binding modes.
Malgieri et al., "A novel type of zinc finger DNA binding domain
in the agrobacterium tumefaciens transcriptional regulator Ros," Biochemistry, vol.