ExoS is a bifunctional enzyme
with both GTPase-activating activity and ADP-ribosyltransferase activity.
The resulting bifunctional enzyme
can be efficiently produced using Escherichia coli, a factor that is crucial for producing carbohydrate products at a reasonable cost.
Catalase-peroxidase (KatG) is a bifunctional enzyme
degrading H2O2 in bacteria and lower eukaryotes.
Bifunctional enzyme 6'-N-aminoglycoside acetyltransferase-2"-O-aminoglycoside phosphotransferase in Lactobacillus and Pediococcus isolates of animal origin.
Susceptibility to aminoglycosides depends on the Lactobacillus species and bifunctional enzymes have been described in some strains, conferring high resistance levels to gentamicin, kanamycin, tobramycin and netilmicin.
For example, MOTIF software locates the aspartate kinase domain of the bifunctional enzyme at the N terminus of the protein and the homoserine dehydrogenase domain toward the C terminus which contains the conserved sequences in Fig.
coli b0002 bifunctional enzyme closer in sequence to the monofunctional enzymes in yeast and in the plant than to the other biofunctional enzyme in E.