Constructon and mutagenesis of an artificial bicistronic
tick-borne encephalitis virus genome reveals an essential function of the second transmembrane region of protein E in flavivirus assembly.
expression vectors encoding different semaphorins and the yellow fluorescing protein Venus, separated by IRES2 element, were constructed in two steps.
Elliott, "Translation efficiencies of the 5' untranslated region from representatives of the six major genotypes of hepatitis C virus using a novel bicistronic
reporter assay system," Journal of General Virology, vol.
Gjerset, "Enhanced tumor suppression by a p14ARF/p53 bicistronic
adenovirus through increased p53 protein translation and stability," Cancer Research, vol.
The pHIV plasmid is a lentiviral construct for bicistronic
expression of the codon-optimised firefly luciferase (luc2) and ZsGreen (via an IRES link) under the constitutive EF1[alpha] promoter.
Gene therapy involves the transduction of neurons in the sensorimotor cortex using a bicistronic
adeno-associated viral vector (AAV) that encodes and expresses a secretory form of ciliary neurotrophic factor (CNTF) coupled to mCHERRY ([AAV-CNTF.sup.mCherry]).
Cells were cotransfected with 40 [micro]g of a bicistronic
L1 and L2 expression constructs (either HPV 16 L1/L2 (p16L1L2) or HPV 18 L1/ L2 (p18L1L2); kindly provided by Professor Martin Muller, DKFZ, Germany) and 40 [micro]g of a GFP expression packaging plasmid (pfwB) (Addgene number 37329) in two different reactions (for HPV 16 L1/L2 and HPV 18 L1/L2) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, US).
Cyclooxygenase-1 and bicistronic
cyclooxygenase-1/prostacyclin synthase gene transfer protect against ischemic cerebral infarction.
Interestingly, S2 is transcribed as a single message with tat and env (tricistronic message) or alternatively in a bicistronic
message with env alone (Fig.
In addition, a combination of doxorubicin with a-fetoprotein- (AFP-) E1A-IRESE1B bicistronic
cassette derived from Ad5, CV890 showed a synergic antitumor effect in vitro as well as in vivo .
Several of these miRNAs are organized under bicistronic
clusters on the same chromosome (i.e., miR-1-1/133a-2, miR1-2/133a-1, and miR-206/133b) and are transcribed together .
Testing truncated versions with bicistronic
reporter assay, Chan et al.