Following 48 hours, we performed the beta-galactosidase
assay as described below.
coli cells, the addition of lactose induces the expression of the enzyme Beta-galactosidase
; in fruit fly cells, increasing the temperature induces the expression of the so-called heat-shock proteins, a family of proteins committed to the repair or the removal of damaged proteins.
The initial stages of the project will involve in vitro and in vivo approaches to develop an RNA aptamer capable of activating mutant Beta-Galactosidase
. This is a novel use of aptamers, and could have implications later when designing regulatory mechanisms.
The isolates were positive for esculin-hydrolysis, negative for ONPG beta-galactosidase
and unable to grow on MacConkey's agar.
Vitamin D-binding protein (known as Gc) can be converted to the potent macrophage activating factor (GcMAF) by stepwise modification of Gc with beta-galactosidase
of B cells and sialidase of T cells.
Castagna et al., "Regenerative treatment using a radioelectric asymmetric conveyor as a novel tool in antiaging medicine: an in vitro beta-galactosidase
study," Clinical Interventions in Aging, vol.
It is caused by a deficiency of beta-galactosidase
(GLB1), a lysosomal hydrolase that maybe defective with respect to keratan sulfate in Morquio B disease (MBD) or to gangliosides, lactosylceramide, asialofetuin, and oligosaccharides carrying terminal beta-linked galactose and keratan sulfate in GM1-gangliosidosis .
We found statistically significant correlations between increased resistance to 5-fluorocytosine and higher enzymatic activity of alpha-mannosidase; increased resistance to ketoconazole and higher enzymatic activity of trypsin; increased resistance to itraconazole and higher enzymatic activity of beta-glucosidase; increased resistance to fluconazole and higher enzymatic activity of beta-glucosidase; increased susceptibility to ketoconazole and higher enzymatic activity of beta-galactosidase
and beta- glucosidase; increased susceptibility to itraconazole and higher enzymatic activity of valine arylamidase.
MPS type IVB is due to deficiency of beta-galactosidase
SA-[beta]-Gal: Senescence-associated beta-galactosidase
DMEM: Dulbecco's modified Eagle's medium EDA: Extract from Deschampsia antarctica EDTA: Ethylenediaminetetraacetic acid FDG: Fluorescein di-[beta]-galactopyranoside HFF: Human foreskin fibroblasts LmnA/C: Lamin A/C MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetra- zolium bromide PCNA: Proliferating cell nuclear antigen PD: Population doubling SHFF: Senescence human foreskin fibroblasts SIPS: Stress-induced premature senescence SIPSF: Stress-induced premature senescence fibroblasts Sirt: Sirtuin.
Of note, A[sz] did not induce the senescence-associated beta-galactosidase
activity in cultured neurons (data not shown), indicating that SA-[sz]-gal was not required for neuronal senescence in vitro , although it was a classic CS biomarker.[sup],, This finding suggested a characteristic aging pattern of neuronal senescence, i.e., the expression of p16 instead of SA-[sz]-gal in vitro .
Saccharomyces fragilis is described as a homothallic, hemiascomycetous yeast and production of several enzymes among them beta-galactosidase
(Llorente et al., 2000; Dagbagli & Goksungur, 2008).