Following 48 hours, we performed the beta-galactosidase
assay as described below.
coli cells, the addition of lactose induces the expression of the enzyme Beta-galactosidase
; in fruit fly cells, increasing the temperature induces the expression of the so-called heat-shock proteins, a family of proteins committed to the repair or the removal of damaged proteins.
The isolates were positive for esculin-hydrolysis, negative for ONPG beta-galactosidase
and unable to grow on MacConkey's agar.
Vitamin D-binding protein (known as Gc) can be converted to the potent macrophage activating factor (GcMAF) by stepwise modification of Gc with beta-galactosidase
of B cells and sialidase of T cells.
Castagna et al., "Regenerative treatment using a radioelectric asymmetric conveyor as a novel tool in antiaging medicine: an in vitro beta-galactosidase
study," Clinical Interventions in Aging, vol.
It is caused by a deficiency of beta-galactosidase
(GLB1), a lysosomal hydrolase that maybe defective with respect to keratan sulfate in Morquio B disease (MBD) or to gangliosides, lactosylceramide, asialofetuin, and oligosaccharides carrying terminal beta-linked galactose and keratan sulfate in GM1-gangliosidosis .
We found statistically significant correlations between increased resistance to 5-fluorocytosine and higher enzymatic activity of alpha-mannosidase; increased resistance to ketoconazole and higher enzymatic activity of trypsin; increased resistance to itraconazole and higher enzymatic activity of beta-glucosidase; increased resistance to fluconazole and higher enzymatic activity of beta-glucosidase; increased susceptibility to ketoconazole and higher enzymatic activity of beta-galactosidase
and beta- glucosidase; increased susceptibility to itraconazole and higher enzymatic activity of valine arylamidase.
MPS type IVB is due to deficiency of beta-galactosidase
SA-[beta]-Gal: Senescence-associated beta-galactosidase
DMEM: Dulbecco's modified Eagle's medium EDA: Extract from Deschampsia antarctica EDTA: Ethylenediaminetetraacetic acid FDG: Fluorescein di-[beta]-galactopyranoside HFF: Human foreskin fibroblasts LmnA/C: Lamin A/C MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetra- zolium bromide PCNA: Proliferating cell nuclear antigen PD: Population doubling SHFF: Senescence human foreskin fibroblasts SIPS: Stress-induced premature senescence SIPSF: Stress-induced premature senescence fibroblasts Sirt: Sirtuin.
Of note, A[sz] did not induce the senescence-associated beta-galactosidase
activity in cultured neurons (data not shown), indicating that SA-[sz]-gal was not required for neuronal senescence in vitro , although it was a classic CS biomarker.[sup],, This finding suggested a characteristic aging pattern of neuronal senescence, i.e., the expression of p16 instead of SA-[sz]-gal in vitro .
Saccharomyces fragilis is described as a homothallic, hemiascomycetous yeast and production of several enzymes among them beta-galactosidase
(Llorente et al., 2000; Dagbagli & Goksungur, 2008).