Interestingly, the expression of beta actin used as an internal control for protein loading in immune western blot was significantly decreased in VPA rats.
In conclusion, our results have demonstrated the renal toxicity of VPA on vasodilatation, serum biochemical changes, and increased MDA level with low beta actin expression, respectively.
Monoclonal antibody to beta actin was purchased from Santa Cruz Biotechnology, Inc.
Western blots were quantified by densitometry and normalized against beta actin, the loading control.
The CMV early enhancer/chicken beta actin
(CAG) promoter can be used to drive transgene expression during the differentiation of murine embryonic stem cells into vascular progenitors.
Housekeeping genes, such as [alpha]-TUB ([alpha]-Tubulin), [beta]-actin (Beta actin
), 18s rRNA (18s ribosomal RNA), 28s rRNA (28s ribosomal RNA), GAPDH (Glyceraldehyde-3-phosphatedehydrogenase), G6PDH (Glucose-6-phosphate dehydrogenase), [beta]-TUB (Beta-Tubulin), RPL32 (Ribosomal protein L32), EF1-[alpha] (Alpha elongation factor), ELF1-[beta] (beta elongation factor), are usually used as endogenous controls for normalization of qRT-PCR data, but the reference gene expression levels can differ under different environment conditions (Radonic' et al.
Robert Singer and his colleagues generated a transgenic mouse in which genes coding for the structural protein beta actin would, when expressed, yield fluorescently labelled mRNA.
Beta actin mRNA is a highly expressed molecule found in all mammalian tissues.
To examine the expression of tyrosine phosphorylated proteins or beta actin
in all epididymal fluids, the separated proteins on SDS gel were transferred onto nitrocellulose membrane.