Congo dye (4) was freshly prepared by diazotization of benzidine
at 0-5degC with nitrous acid generated in situ by the reaction of sodium nitrite and hydrochloric acid.
test was applied to confirm presence of blood in urine and further urine was centrifuged to rule out haematuria.
Biodegradation of benzidine
based dye Direct Blue-6 byPseudomonas desmolyticum NCIM 2112.
Certain aromatic amines such as benzidine
are hazardous to human health.
Finally, hydrogen peroxide and tetramethyl benzidine
(TMB) were added and the plates were incubated for 15 min at room temperature.
The carcinogenicity and metabolism of azo dyes, especially those derived from Benzidine
Manufacturers of very toxic products such as asbestos, arsenic, trioxide, benzidine
based dyes, and some pesticides,
Detection was accomplished with the Dako EnVision System (Carpinteria, California), and the expression of both H3K9me3 and H3K9Ac was detected with the diamino benzidine
chromogen system (Dako).
The kit used in this study is a solid-phase sandwich ELISA using 2 high specific antibodies and tetra methyl benzidine
(TMB) as a coloring agent (Chromogen).
The wells were washed 5 times with PBS-T, and 100 [micro]L tetramethyl benzidine
solution (Kem-En-Tec) was added to each well as a substrate, followed by incubation in the dark for 30 min at room temperature.
The slides were then washed three times with PBS and thereafter sections were stained with 3, 3\'-Diamino benzidine
(DAB) substrate solution.
According to Jane Hersey, Director of the Feingold Association, which works with children's food-caused health and behavior issues, Red Dye #3 has been shown to cause cancer and three other dyes (Red #40, Yellow #5, and Yellow #6) are contaminated with low levels of known cancer-causing compounds, such as benzidine