In addition, plant extracts are categorized in generally recognized as safe for human consumption; therefore, numerous studies have evaluated the efficacy of EO and essential oil compounds (EOC) with the aim of replacing antibiotic rumen modulators such as monensin, using an in vitro batch culture
systems [2,5-7] and continuous culture systems [8-10].
The inhibitory effect on microbial fermentation confirmed potent antimicrobial activity of EO (Reuter et al., 1996; Davidson and Naidu, 2000) and agree with previous in vitro batch culture
reports where high doses of EO have been tested (Busquet et al., 2005; Busquet et al., 2006; Castillejos et al., 2007; Durmic et al., 2014; Jahani-Azizabadi et al., 2011).
An asset of GREENSEA is the diversity of its culture systems: batch culture
systems (Annular and disposable bags, photobiore-actors), or continuous culture systems (tubular photobioreactor), representing a total of 100 m3 and spanning over 800m2.
To determine if medium components were limiting SF-MSC growth in suspension culture, the effect of medium replenishment was evaluated by culturing cells in either batch culture
, where no medium replenishments were made, or in cyclic fed-batch cultures, where a 50% medium replenishment was performed on days 3, 6, and 9.
Based on she (9) by batch culture
electrolytic stimulation of cell culture process research, The suitable current intensity is 10 ma in DC.
Callus cells were transferred to suspension and a batch culture
of suspension was initiated.
Other authors report a 56.5% improvement in L(+) lactic acid production using exponential feeding compared to traditional batch culture
, achieving 157.5 g/L of L(+) lactic acid and productivity of 1.88 g/L - h by Lactobacillus casei after 84 h of fermentation .
After confirmation of both methods giving nearly the same results, PHB content from bacterial batch culture
was assayed using the spectrophotometric method (Supplementary Table S4).
The fermentation process can be one of three types: either batch culture
(all substrate is present at the beginning of the reaction and nothing is added or removed from the fermentor during the reaction); fed-batch culture (fresh medium is added during the reaction to prevent nutrient depletion, but nothing is removed); and continuous culture (fresh medium is added during the reaction while old medium is removed) .
Experiments were maintained in batch culture
mode and the culture parameters periodically measured for 28 days.
acetobutylicum, batch culture
fermentation was run using 50g/L date fruit in P2 medium at 35[degrees]C for 72 h.