After this period, the samples were washed in distilled water and ethanol 50%, and with a brush the two epidemial surfaces were separated, stained with basic fuchsin
(Roeser, 1962), and mounted in glycerine jelly.
The variation of adsorption of basic fuchsin dye with contact time in the low and high concentration range is shown in Fig.
5-20 mg/L and high concentration range of 100-1000 mg/L of basic fuchsin dye in Fig.
For comparison of these two types of regression, both linear and non-linear fitting was carried out for basic fuchsin dye in the low concentration range for pseudo first order and pseudo second order kinetics.
Interpretation & conclusions: Sputum samples can be stored for up to seven days in the sputum container with phenol ammonium sulphate basic fuchsin solution.
We have earlier shown that sputum in its containers can be stained (11), in an hour, by phenol ammonium sulphate basic fuchsin solution and its smear can then be decolourised and counter-stained for detection of acid-fast bacilli (AFB) (12,13).
8% basic fuchsin
for safranin weakly staining Gram negatives Addition of fast green and Improved contrast between tartrazine before counter- Gram negatives and back- staining with safranin ground
Therefore, the objectives of the present study were (i) to stain the sputum samples in their containers with phenol ammonium sulphate basic fuchsin solution and to decolourise and counter-stain the stained sputum smears for detection of AFB (henceforth called pot method, and (ii) to compare the proportion of AFB positives obtained in pot and standard ZN methods.
Therefore, phenol ammonium sulphate basic fuchsin stained sputum smears can be made on work-benches in any clinical laboratories.
1966), methylene blue/ basic fuchsin
(Bennett et al.
ceti, B1 to B6 ** + + - + + + (+) (-) (-) (-) Growth on dyes, [micro]g/ mL ([double dagger]) Basic fuchsin
O safranin Omp2b restriction Species, strain ([section]) 20 100 pattern B.
Acetic acid, Acetone, Acid fuschin, Ammonium alum(aluminium ammonium sulphate), Ammonium hydroxide, Ammonia solution, Aniline blue, Basic fuchsin
, Bismark brown, Tips for micropipette 100-1000 micro litre, Calcium chloride, Charcoal, Chloroform, Citric acid, Congo red, Copper sulphate, Chloral hydrate, Crystalline phenol, D.