The karyotype formula, chromosomal location of 5S rDNA, and base composition
of supernumerary chromosome of I.
8 which was selected from table 2 as a base composition
for third stage melting.
The frequency of occurrence of each base composition
was defined as the frequency that each expected base composition
occurred over the set of 968 sequences in the database.
Table 2 shows nucleotide base composition
and base composition
The codon usage of the 13 protein-coding genes and the base composition
of 37 genes for three species were analyzed using the program MEGA 4.0.
Because size and base composition
influence the retention of single-stranded DNA in DHPLC (4), multiplexing of two or more minisequencing reactions requires empirical determination of the retention times for the primers and extension products in DHPLC.
If codon usage is selected, then mutation pressure will not be the sole determinate of base composition
in third positions, and the story told by Osawa becomes more complicated.
Alloying elements such as Cu, Ni and Mo are added to the base composition
individually or in combination.
and codon usage were calculated with DNAStar software.
Phylogenetic trees were produced with a maximum likelihood method incorporating the GTR+ [GAMMA]+I model of nucleotide substitution, with the general time-reversible (GTR) substitution matrix, the base composition
, the gamma ([GAMMA]) distribution of among-site rate variation, and the proportion of invariant sites (I) all estimated from the data.
Because of the length and base composition
of the probe, the melting points were higher compared with the apoE probes.