Plant regeneration through adventitious shoots and somatic embryos was achieved using different explant types, basal medium
or growth regulators on similar and close relative Muscari and Bellevalia species Bellevalia romana (Lupi et al., 1985), Muscari armeniacum Leichtlin ex Baker (Suzuki and Nakano, 2001), Muscari comosum var.
The chemical structure of cassava starch and the compatibilizing agents containing functional groups along the chain make the blend more susceptible to attack by the basal medium
and microorganisms, since these bonds are susceptible to hydrolytic degradation, as has been reported by other authors .
and replaced with basal medium
with DMEM glutamax, enriched with Platelet rich plasma lysate and antibiotics PenStrepGibco[TM] containing Streptomycin Sulfate 100 iG/mL and Penicillin G Sodium Sulfate 100U/mL (Thermofisher Scientific).
The pH of the basal medium
was adjusted by adding 0.1N NaOH or 0.1N HCl and incubated at 27 [+ or -] 2[degrees]C temperatures for 20 days at stationary condition (16).
After culture, the medium was replaced with RPMI 1640 basal medium
supplemented with 10% WST-1 reagent (Roche, Mannheim, Germany).
For enrichment culture and isolation, basal medium
was prepared with the modification from Sowers and Schreier  and contained the following compounds (per liter distilled water): KCl, 0.335 g; [MgCl.sub.2] - [6H.sub.2]O, 2.75 g; [MgSO.sub.4] - [7H.sub.2]O, 3.450 g; [NH.sub.4]Cl, 0.25 g; [CaCl.sub.2] - [2H.sub.2]O, 0.14 g; [K.sub.2][HPO.sub.4], 0.14 g; NaCl, 18.0 g; trace element solution, 10.0 mL; vitamin solution, 10.0 mL; Fe[([NH.sub.4]).sub.2] [([SO.sub.4]).sub.2] [7H.sub.2]O, 0.002 g; [NaHCO.sub.3], 5.0 g; methanol, 5.0 mL; sodium acetate, 2.5 g; yeast extract, 2.0 g; trypticase peptone, 2.0 g; resazurin, 0.001 g; cysteine hydrochloride, 0.5 g; and [Na.sub.2]S - [9H.sub.2]O, 0.5 g.
Percent shoot regeneration from leaf explant was highest (86.66%) followed by in vitro source of explant (83.65%), B5 basal medium
(83.20%) or 2.27 uM TDZ (73.70%) after 60 days of initial culture.
Two days post-confluence (Day 0), the cells were induced to differentiate using an adipogenic cocktail [1 mM dexamethasone, 1 mg/mL insulin, and 0.5 mM methylisobutylxanthine, (DIM)] added to a basal medium
[DMEM with 10% (vol/vol) FBS and penicillin, streptomycin, and amphotericin].
With the exception of pyruvate and lactate, all test compounds induced a significant stimulation of insulin secretion when compared to the preperifusion phase with basal medium
The excised shoot tips or leaves discs (1x1 cm2), stem discs (1-2 cm) and root discs (1-2 cm) of cotton seedlings were cultured on MS basal medium
(Murashige and Skoog, 1962) supplemented with various concentrations of BAP i.e.
This was carried out with serial diluted sample of 1g of soil sample from each location, plated on sterile M9 basal medium
 and incubated at 30C for 48 hrs.
Preparation of basal groundnut broth(groundnut basal medium