Single factor experiment was used to select the best carbon and nitrogen sources, barbital
, pH, and moisture levels.
Un pL de extracto fue sometido a un campo electrico de 90V por 35 min, utilizando para ello buffer barbital
pH 8,6 Corning (N- de catalogo 470180).
For these assays, thyroid hormone standards (0.22-160 ng/mL [T.sub.4]; 0.06-10 ng/mL [T.sub.3]) were prepared in barbital
The animals were anesthetized with 1% sodium barbital
and fastened abdomen-laterally.
Total [T.sub.4] was measured in 5 [micro]L of rat serum using a barbital
Calibrators were prepared from synthetic proCNP 1-10-Ala-Ala-AlaAla-Tyr in 20 mmol/L barbital
buffer (pH 8.4) containing 1.1 g/L bovine serum albumin and 0.6 mmol/L thiomersal.
Fujimori H (1965) Potentiation of barbital
hypnosis as an evaluation method for central nervous system depressant.
Electrophoretic patterns of samples were determined on 10% agarose gel films (Titan Gel Lipo; Helena) in a 0.06 mol/L barbital
buffer (pH 8.6) for 30 min at 90 V.
The buffers and solutions used regularly were as follows: phosphate-buffered saline [PBS; 10 mmol/L sodium phosphate buffer (pH 7.4) containing 140 mmol/L NaCI]; PBS-EDTA (PBS-E), PBS containing 200 [micro]mol/L EDTA; blocking buffer, PBS containing 20 g/L BSA, 0.5 mL/L Tween 20, and 200 [micro]mol/L EDTA; washing buffer (PBS-Tween), PBS containing 0.5 mL/L Tween 20; barbital
buffer (Sigma), which was prepared as suggested by the manufacturer; and ELISA stop solution, 1 mol/L [H.sub.2]S[O.sub.4].
Briefly, electrophoresis in the first dimension was performed in 0.75% agarose gels in 50 mmol/L barbital
buffer (pH 8.6).
Electrophoresis and immunoelectrophoresis were carried out in agarose gels using a barbital
buffer (pH 8.6; ionic strength = 0.05) for 30 min at 100 V (Paragon immunofixation electrophoresis method; Beckman).
Two (3.2-mm (1/8-inch) diameter disks were punched from dried blood-spot calibrators or controls and eluted in 100 [micro]L of 0.075 mol/L sodium barbital
buffer (pH 8.6) containing 0.5 g/L anilinonaphthalenesulfonic acid and 0.5 g/L sodium azide by sonication at room temperature for 30 min The volume of blood per 3.2-mm disk was (3 [micro]L (3).