In addition, a system of Agrobacterium- mediated transient expression of b-glucuronidase (GUS) reporter gene in late unicellular and bicellular pollen was also established.
In addition, a system of Agrobacterium-mediated transient expression of b-glucuronidase (GUS) reporter gene was also established and transgenic research, achieved initial success.
ABSTRACT: This study is carried to optimize Arabidopsis transformation protocol using b-glucuronidase (GUS) gene as a reporter gene to analyse gene expression in heterologous system.
Here we report the optimization of Agrobacterium mediated stable transformation of Arabidopsis using b-glucuronidase (GUS; Jefferson et al.
coli produce b-glucuronidase
enzyme which enables them to hydrolyse MUG, resulting in the release of a fluorogenic compound.
1991, "Stable transformation of Sorghum bicolor protoplasts with chimeric neomycin phosphotransferase II and b-glucuronidase
4 U/L B-glucuronidase (Sigma G7396) and incubated the plates with shaking for 60 min at room temperature.
By incorporating B-glucuronidase in the DELFIA assay buffer (pH 7), this enzyme treatment could be performed as an integral part of the urinary daidzein, genistein, equol, and (nonextracted) serum daidzein assays.
For example, B-glucuronidase
is an important enzyme that releases toxic aglycones in the colon.
Enzyme-potentiated hyposensitization: Effects of glucose, glucosamine, N-acetylamino-sugars and gelatin on the ability of B-glucuronidase
to block the anamnestric response to antigen in mice.
Its first two commercial products were avidin and B-glucuronidase
for use in the diagnostic and research chemical markets.
The study also suggested that the enzyme B-glucuronidase
, which has been implicated in colon cancer, is reduced in the colons of people with FOS-supplemented diets.