ANCAs are auto-antibodies directed against azurophilic
proteins within neutrophils, including perinuclear ANCAs (p-ANCAs) and cytoplasmic ANCAs.
Diagnosis can be made with a simple peripheral smear for the classic giant azurophilic
granules, which are peroxidase-positive, in all granule-containing cells including the peripheral blood and bone marrow.
The urine strip test for white blood cells detects leucocyte esterase, which is present in azurophilic
granules of leucocytes.
Myeloperoxidase is an enzyme found in azurophilic
granules of neutrophils (and less in monocytes) that also contain heme, but rather than being red-brown, it is yellow-green.
MPO is abundantly stored and expressed in primary azurophilic
granules of neutrophils.
(primary) granules contain MPO, defensins, and proteinases.
MPO, a member of the heme peroxidase superfamily, is contained in azurophilic
granules in neutrophils and monocytes.
Myeloperoxidase is a 150-165-kDa molecule synthesized during myeloid differentiation that constitutes the major component of the neutrophil azurophilic
In contrast with Santos (2001) for carijo hawk (Buteo magnirostris), in this study, two azurophilic
granules were not observed in one of the thrombocyte poles in rhea.
Identification of cell abnormalities--such as the large azurophilic
granules in toxic granulation, asynchronous maturation, micromegakaryocyte identification or quantitating variant lymphs--still requires a skilled human eye.
The hydrogen peroxide in the chloride ions is reduced to hypochlorous acid, a toxic and strong oxidant, via the MPO enzyme found in the azurophilic
granules of neutrophils (ZIMMERMAN & GRANGER, 1992).
In order to confirm the cellular response in the collected synovial fluid after zymosan injection (6h), it was also determined the MPO activity, an enzyme found primarily in the azurophilic
granules of the neutrophils and has been widely used as a biochemical marker of granulocyte infiltration into various tissues.