Cathepsin G is one of the three serine proteases (along with proteinase-3 and elastase) that are contained in primary (azurophil
) granules of neutrophils and involved in clearance of internalized pathogens, proteolytic modification of chemokines and cytokines, and shedding of cell surface receptors .
Hieblot et al., "Presence of proteinase 3 in secretory vesicles: evidence of a novel, highly mobilizable intracellular pool distinct from azurophil
granules," Blood, vol.
Importantly, granule contents are also released according to a hierarchy, with secretory granules being the most readily exocytosed and azurophil
granules only undergoing partial exocytosis .
Kew, "Upregulation of vitamin D binding protein (Gc-globulin) binding sites during neutrophil activation from a latent reservoir in azurophil
granules," Molecular Immunology, vol.
Furthermore, [alpha]-defensin has not been detected in mouse neutrophils, (32) while human [alpha]-defensin is a major component of azurophil
granules of human neutrophils.
Identification of constituents of human neutrophil azurophil
granules that mediate fungistasis against histoplasma capsulatum.
Neutrophil elastase, a serine protease, is normally located within azurophil
granules of neutrophils and degrades Gramnegative bacteria and some other proteins such as coagulation proteins, growth factors, extracellular matrix proteins, immunoglobulins, and complement proteins, as well as PML-RAR[alpha] fusion protein .
Differences in enzyme content of azurophil
and specific granules of polymorphonuclear leukocytes.
There is a strong and specific association of WG with presence of anti-neutrophil cytoplasmic antibodies (ANCA) to a defined target antigen, proteinase 3 (PR3-ANCA), which is present within primary azurophil
granules of neutrophils and lysozymes of monocytes (2).
Hypochlorous acid generated by the concerted activity of membrane-bound NADPH oxidase and azurophil
granule myeloperoxidase of the neutrophil produces luminol chemiluminescence.
HNP1-3 are primarily stored in the azurophil
granules of neutrophils and then released into circulation and infection sites in response to infectious pathogens .
(or primary) granules are the first to be produced and contain MPO and a spectrum of neutrophil serine proteases (NSPs): cathepsin G (CG), neutrophil elastase (NE), proteinase 3 (PR3), and the recently discovered neutrophil serine protease-4 (NSP4) [30, 90].