Non-Alcoholic, Azocarmine Stain (X400) Alpha cells, B--Beta cells
Alcoholic, Mallory Heidenhain's Azocarmine Stain (X400) Alpha cells, B--Beta cells
Azocarmine and orange G staining: The samples stained by 0.5% azocarmine for 30 s, water rinsing, stained by 2% orange G (orange G 2 g + phosphotungstic acid 1 g + water 100 ml) for 30 min.
The results of azocarmine and orange G staining could demonstrate the difference of two types of acidophil cells: Orange somatotropic cells and nacarat lactotrophic cells (Figs.
Three-micrometer-thick sections of paraffin-embedded kidneys were then subjected to hematoxylin and eosin (H&E) staining by a routine procedure (Meyer's hematoxylin staining, followed by eosin Y staining) and azan staining by a routine procedure (Mordant, Mallory's azocarmine
G solution, 5% phosphotungstic acid solution, and Mallory's aniline blue orange G stain solution).
Next, the sections were stained in azocarmine
for 1 hr and then rinsed in distilled water.
For morphometric analysis, individual flukes were washed, three times, in PBS and fixed and stained in FAAL (formalin, azocarmine
, alcohol, and lactic acid) solution followed by mounting with a medium containing poly vinyl alcohol .
Among the protocols reported in the literature for this group, highlights the trichromics of Mallory (Pantin, 1948), Azocarmine
(Humason, 1967), Heidenhain Azan (Presnell & Schreibman, 1997) and the Nuclear Red Picroindigocarmine (Romeis, 1948); the latter being recommended by Stotz (1981).
The sections, which included three spatial planes, were stained with haematoxylin-eosin (HE), azocarmine
, Bielchowsky and Masson's trichrome dye .
In contrast to those cells, however, Rhinodrilus priollii glands displayed granules that stained with both alcian-blue (outer granule) and azocarmine
(inner granule) in Masson's stain, suggesting a proteinaceous core surrounded by a glycosylated external layer.
The 40-[micro]m-thick sections were stained with Mayer's haematoxylin and 0.15% basic fuchsine, and the 2[micro]m-thick sections were stained with Mayer's haematoxylin, azocarmine
solution, and anilineblue-orange G.
Sections, taken every 200-250 [micron]m, were stained with Heidenmhain's Azocarmine
Aniline Blue (Luna 1968, Acosta and Zea 1997).