A series of azo dyes used in preparing tissue stains.
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Non-Alcoholic, Azocarmine Stain (X400) Alpha cells, B--Beta cells
Alcoholic, Mallory Heidenhain's Azocarmine Stain (X400) Alpha cells, B--Beta cells
Azocarmine and orange G staining: The samples stained by 0.5% azocarmine for 30 s, water rinsing, stained by 2% orange G (orange G 2 g + phosphotungstic acid 1 g + water 100 ml) for 30 min.
The results of azocarmine and orange G staining could demonstrate the difference of two types of acidophil cells: Orange somatotropic cells and nacarat lactotrophic cells (Figs.
Three-micrometer-thick sections of paraffin-embedded kidneys were then subjected to hematoxylin and eosin (H&E) staining by a routine procedure (Meyer's hematoxylin staining, followed by eosin Y staining) and azan staining by a routine procedure (Mordant, Mallory's azocarmine G solution, 5% phosphotungstic acid solution, and Mallory's aniline blue orange G stain solution).
Next, the sections were stained in azocarmine for 1 hr and then rinsed in distilled water.
For morphometric analysis, individual flukes were washed, three times, in PBS and fixed and stained in FAAL (formalin, azocarmine, alcohol, and lactic acid) solution followed by mounting with a medium containing poly vinyl alcohol [17].
Among the protocols reported in the literature for this group, highlights the trichromics of Mallory (Pantin, 1948), Azocarmine (Humason, 1967), Heidenhain Azan (Presnell & Schreibman, 1997) and the Nuclear Red Picroindigocarmine (Romeis, 1948); the latter being recommended by Stotz (1981).
The sections, which included three spatial planes, were stained with haematoxylin-eosin (HE), azocarmine, Bielchowsky and Masson's trichrome dye [27].
In contrast to those cells, however, Rhinodrilus priollii glands displayed granules that stained with both alcian-blue (outer granule) and azocarmine (inner granule) in Masson's stain, suggesting a proteinaceous core surrounded by a glycosylated external layer.
The 40-[micro]m-thick sections were stained with Mayer's haematoxylin and 0.15% basic fuchsine, and the 2[micro]m-thick sections were stained with Mayer's haematoxylin, azocarmine solution, and anilineblue-orange G.
Sections, taken every 200-250 [micron]m, were stained with Heidenmhain's Azocarmine Aniline Blue (Luna 1968, Acosta and Zea 1997).