Among the protocols reported in the literature for this group, highlights the trichromics of Mallory (Pantin, 1948), Azocarmine
(Humason, 1967), Heidenhain Azan (Presnell & Schreibman, 1997) and the Nuclear Red Picroindigocarmine (Romeis, 1948); the latter being recommended by Stotz (1981).
In contrast to those cells, however, Rhinodrilus priollii glands displayed granules that stained with both alcian-blue (outer granule) and azocarmine
(inner granule) in Masson's stain, suggesting a proteinaceous core surrounded by a glycosylated external layer.
15% basic fuchsine, and the 2[micro]m-thick sections were stained with Mayer's haematoxylin, azocarmine
solution, and anilineblue-orange G.