Prior to specimen inoculation 0.1 mL of PANTA supplement (polymyxin B 50 [micro]g/mL, amphotericin B 5 [micro]g/mL, nalidixic acid 20 [micro]g/mL, trimethoprim 5 [micro]g/mL and azlocillin
10 [micro]g/mL) was added to each BACTEC 12B vial.
Antibiotics discs used in the experiment included Ticarcillin (70ug), Cefixime (5ug), Cloxacillin (1ug), Sulphaphenazol (200ug), Gentamicine (10ug), Amoxicillin (30ug),Vancomycin (30ug), Ceftaxime (30ug), Ceftazidine (30ug), Amoxicillin (25ug), Cefuroxime (30ug), Ciprofloxacillin (5ug), Ampicillin (10ug), Oxacillin (1ug), Amicacin (30ug), Azlocillin
(75ug), Piperacillin Tazabactam (100:10 ug), Ticarcillin Calvanic acid (75:10ug), Triple Sulphas (300ug), Cinoxacin (100ug), Mupiracin (5ug), Spectinomycin (100ug), Chloramphenicol (25ug), Trimethoprim (30ug).
For preparing the MGIT culture tubes (Becton Dickinson, Maryland, USA), a vial of 15 mL OADC enrichment (oleic acid, albumin, dextrose and catalase) was well-mixed with a vial of PANT antibiotics (polymixin B, amphotericin B, nalidixic acid, trimethoprim, azlocillin
) before culturing and 0.8 ml was added to each MGIT culture medium by an insulin syringe.
To each well 700 [micro]L of Middlebrook 7H9 broth, 100 [micro]L mixture of polymyxin B, amphotericin B, nalidixic acid, trimethoprim, and Azlocillin
(PANTA; BBL MGIT PANTA Antibiotic Mixture; BD), 100 [micro]L of solutions of INH 1 [micro]g/mL or RIF 10 [micro]g/mL and 100 [micro]L of the processed specimen was added, thus obtaining a final volume of 1mL per well and critical concentrations of 0.1 [micro]g/mL INH and 1 [micro]g/mL RIF.
Austin, TX, August 27, 2015 --(PR.com)-- Bioo Scientific's MaxSignal[R] Beta-Lactam ELISA Test Kit has been validated for the screening of a wide range of beta-lactam antibiotics including ampicillin, penicillin G, amoxicillin, azlocillin
, piperacillin, nafcillin, cloxacillin, oxacillin, cefoperazone, ceftiofur and other beta-lactams in a number of food matrices.
Antibiotic prophylaxis before extracorporeal shock wave lithotripsy by single-shot application of azlocillin
. Chemioterapia 1987;6(2 Suppl):607-9.
 PANTA comprises 5 antimicrobial agents: polymyxin B, amphotericin B, nalidixic acid, trimethoprim and azlocillin
. The addition of this antibiotic mixture has been found to increase rather than decrease MTB culture yields.
The bottle also contains enrichment broth with glucose, glycerin, oleic acid, pyridoxal, catalase, albumin, polyoxyethylene 40 stearate, azlocillin
, nalidixic acid, trimethoprim, polymyxin B and amphotericin B.
Auramine-rhodamine fluorescent AFB smear was read and the specimen inoculated onto Lowenstein-Jensen (LJ) slant and MGIT (Becton Dickinson Diagnostic Instruments Systems, Sparks, MD, USA) supplemented with oleic acid-albumin-dextrose- catalase (OADC) and PANTA (polymyxin B, amphotericin B, nalidixic acid, trimethoprim and azlocillin
The antibiotics included were piperacillin (100[micro]g), cefotaxime (30[micro]g), ceftazidime (30[micro]g), cefoperazone (75[micro]g), tobramycin (30[micro]g), ceftriaxone (30[micro]g), amikacin (30[micro]g), netilmicin (30[micro]g), ofloxacin (5[micro]g), ciprofloxacin (5[micro]g), imipenem (10[micro]g), mezlocillin (75[micro]g), azlocillin
(75[micro]g), ticarcillin (75[micro]g).
The older antipseudomonal penicillins (carbenicillin, ticarcillin) have largely been replaced by newer compounds (piperacillin, mezlocillin, azlocillin
) which are thought to be more potent in vitro against P.
Aliquots (0.5 mL) were transferred to 2 mycobacterial growth indicator tubes, 1 of which contained polymyxin, azlocillin
, nalidixic acid, trimethoprim, and amphotericin B.