The pressure wave in the axoplasm
is governed by a 1D Navier-Stokes model:
By isolating proteins from axoplasm
, they have determined those essential to movement.
The Schwann cell lamina enveloped each nerve fiber and in the axoplasm
was noted the meshwork of neurofilaments, mitochondria and neurotubules (Figs.
About 3-10 [micro]l of pure axoplasm
, uncontaminated by sheath or glial cells, can be collected from a single giant axon, depending on size, making it possible to collect up to 300-1000 [micro]l of axoplasm
from 10 squid (2 axons per squid).
Preliminary experiments were performed in which the His-kinesin tail fragment was added to extruded axoplasm
from the squid giant axon (Fig.
In this study we show that the globular tail fragment of myosin-V displaces native myosin-V from vesicles isolated from squid brain and blocks vesicle transport on actin filaments in axoplasm
from the squid giant axon.
5 mg/ml) was added to axoplasm
in the presence of 5 mM ATP, and the sample was warmed to 24[degrees]G.
We have developed novel methodology for isolating them from axoplasm
Direct measurements of axoplasm
ic resistance further supported this hypothesis.
Therefore we plan to produce squid recombinant proteins to determine whether vesicle transport is inhibited in axoplasm
supports the retrograde axonal transport of Herpes simplex virus.
was extruded with a roller [ILLUSTRATION FOR FIGURE 1A OMITTED].