For fast kinetics parameters measurement, affinity interactions between biotin and
avidin were characterized by the association rate constant [k.sub.a], the dissociation rate constant [k.sub.d], and the equilibrium association constant [K.sub.eq].
The first was due to the Fe-O stretching mode of the [Fe.sub.3][O.sub.4] MNPs, and the second and third were associated with the [alpha]-helical and [beta]-folded types of secondary structure of
avidin, respectively [34, 35].
After adding
avidin to the modified S1-ADP-Vi, the complex of actin-tropomyosin and S1-ADP-Vi, of which the biotinylated ADP was associated tightly with
avidin, was observed by electron microscopy.
In the second method, an unlabeled
avidin film and a ds-DNA film were assembled on the semiconductor electrode.
The activity of acetyl-CoA carboxylase in mammary gland nuclei-free homogenates prepared in the presence and absence of
avidin indicates that polymerization/ depolymerization are not solely responsible for the modification of activity (Table 4).
Another question that often comes up in discussions about home-prepared canine diets has to do with the
avidin in raw egg whites causing a biotin deficiency, but there is no need to worry.
Ark Therapeutics has developed Scavidin, a version of a naturally occurring molecule called
avidin, which recognises the cancer-fighting drug used in radiotherapy and chemotherapy and then "shuttles" it back and forth in the body, picking up the cancer drugs and taking them to the heart of the tumour.
Ark Therapeutics has developed Scavidin - a version of a naturally occurring molecule called
avidin - which recognises the cancer-fighting drug used in radiotherapy and chemotherapy and then 'shuttles' it back and forth in the body, picking up the cancer drugs and taking them to the heart of the tumour.
These technical questions were examined several years ago with a proof-of-principle product,
avidin.
Avidin is a protein normally found in chicken egg whites and binds biotin (DeLange and Huang, 1971).
Further, the lab has created a hybrid material by combining the quantum dot with protein
avidin and biomolecule biotin.
Avidin was adsorbed to glass beads that had been derivatized and biotinylated.
We purchased antimurine IL-4 monoclonal antibody (mAb) clone 11B11 from Hazleton (Hazleton, PA, USA); biotin conjugated anti-IL-4 mAb clone BVD6-24G2 from Pharmingen (San Diego, CA, USA); horseradish peroxidase (HRP)-conjugated
avidin from ICN Pharmaceuticals Inc.
