For fast kinetics parameters measurement, affinity interactions between biotin and avidin
were characterized by the association rate constant [k.sub.a], the dissociation rate constant [k.sub.d], and the equilibrium association constant [K.sub.eq].
The first was due to the Fe-O stretching mode of the [Fe.sub.3][O.sub.4] MNPs, and the second and third were associated with the [alpha]-helical and [beta]-folded types of secondary structure of avidin
, respectively [34, 35].
After adding avidin
to the modified S1-ADP-Vi, the complex of actin-tropomyosin and S1-ADP-Vi, of which the biotinylated ADP was associated tightly with avidin
, was observed by electron microscopy.
In the second method, an unlabeled avidin
film and a ds-DNA film were assembled on the semiconductor electrode.
The activity of acetyl-CoA carboxylase in mammary gland nuclei-free homogenates prepared in the presence and absence of avidin
indicates that polymerization/ depolymerization are not solely responsible for the modification of activity (Table 4).
Another question that often comes up in discussions about home-prepared canine diets has to do with the avidin
in raw egg whites causing a biotin deficiency, but there is no need to worry.
Ark Therapeutics has developed Scavidin, a version of a naturally occurring molecule called avidin
, which recognises the cancer-fighting drug used in radiotherapy and chemotherapy and then "shuttles" it back and forth in the body, picking up the cancer drugs and taking them to the heart of the tumour.
Ark Therapeutics has developed Scavidin - a version of a naturally occurring molecule called avidin
- which recognises the cancer-fighting drug used in radiotherapy and chemotherapy and then 'shuttles' it back and forth in the body, picking up the cancer drugs and taking them to the heart of the tumour.
These technical questions were examined several years ago with a proof-of-principle product, avidin
is a protein normally found in chicken egg whites and binds biotin (DeLange and Huang, 1971).
Further, the lab has created a hybrid material by combining the quantum dot with protein avidin
and biomolecule biotin.
was adsorbed to glass beads that had been derivatized and biotinylated.
We purchased antimurine IL-4 monoclonal antibody (mAb) clone 11B11 from Hazleton (Hazleton, PA, USA); biotin conjugated anti-IL-4 mAb clone BVD6-24G2 from Pharmingen (San Diego, CA, USA); horseradish peroxidase (HRP)-conjugated avidin
from ICN Pharmaceuticals Inc.