pseudomallei 82 (a purine auxotroph
) required longer incubation (5 d) before a turbidity increase was evident.
Li et al., "Tumor-targeting bacterial therapy with amino acid auxotrophs
of GFP-expressing Salmonella typhimurium," Proceedings of the National Academy of Sciences of the United States of America, vol.
Microbial production of L-glutamic acid by glycerol auxotrophs
Nakamaya  cultured Brevibacterium and Corynebacterium auxotrophs
on 20-40oC and reported maximum lysine production 17- 39g/L at 30oC that is also in support of our optimized temperature.
The present study focused on the development of auxotroph
and over production of lysine in stirred tank fermentation, after releasing the various metabolic control mechanism and utilizing three different media.
Differential expression of Met auxotroph
, Escherichia coli DL41 (DE3) pCOCK, has revealed proteomic variation in cells forced to use TeMet in the translation of proteins.
For example, auxotroph
mutants are easy to maintain and observe on selective media and are strongly selected against on restrictive media (Welden & Hossler, 2003; Krist & Showsh, 2007).
The yeast morphotype but not the mold, for example, has a cysteine dioxygenase activity and is a cysteine auxotroph
. To begin studies on the role of sulfur metabolism in dimorphism, we are isolating genes important in the central sulfur metabolic pathway.
coli NK3, a cysteine auxotroph
. This mutant lacks the gene for OAS-TL and therefore cannot grow in medium without supplemental cysteine.
When a polyamine auxotroph
is grown under conditions of polyamine starvation, cadaverine is present in the perchloric acid-extractable pool.
Paradoxically, single and double auxotroph
strains had lower mean MICs than the 2 nonauxotroph strains.
As a result, a conditional promoter strategy driving the expression of AroB (chorismate synthase) was used to obtain aromatic amino acid auxotroph
strain, which was unable to grow in medium containing the three aromatic amino acids and displayed attenuated virulence in pulmonary and systemic murine infection models.