autoradiograph

(redirected from autoradiographed)
Also found in: Dictionary, Thesaurus.

autoradiograph

 [aw″to-ra´de-o-graf]
the film produced by autoradiography.
Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved.

au·to·ra·di·o·graph

(aw'tō-rā'dē-ō-graf),
Image of the distribution and concentration of radioactivity in a tissue or other substance made by placing a photographic emulsion on the surface of, or in proximity to, the substance.
Synonym(s): autoradiogram
Farlex Partner Medical Dictionary © Farlex 2012

autoradiograph

(ô′tō-rā′dē-ō-grăf′)
n.
An image recorded on a photographic film or plate produced by the radiation emitted from a specimen, such as a section of tissue, that has been treated or injected with a radioactively labeled isotope or that has absorbed or ingested such an isotope. Also called autoradiogram.

au′to·ra′di·o·graph′ic adj.
au′to·ra′di·og′ra·phy (-ŏg′rə-fē) n.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.

autoradiogram

A photograph produced by exposing undeveloped film to a radiolabeled molecule (e.g. DNA, RNA or protein), typically in the context of a restriction enzyme digested blot.
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

au·to·ra·di·o·graph

(aw'tō-rā'dē-ō-graf)
Image of the distribution and concentration of radioactivity in a tissue or other substance made by placing a photographic emulsion on the surface of, or in close proximity to, the substance.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

autoradiograph

a photographic representation of radioactive areas in a tissue. Tissues labelled with an ISOTOPE (e.g. 14C) are placed on a photographic plate which is irradiated in the same way as photographic film is exposed to light. Radioactive areas can be seen in the photographs.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
For the novel Southern blot analysis, 10 [micro]g of genomic DNA was digested with the restriction endonucleases AseI and NdeI, resolved on a 0.65% agarose gel, blotted on a Hybond-[N.sup.+] membrane (Amersham Pharmacia Biotech AB), hybridized with an [[[alpha]-.sup.32]P] dCTP-labeled probe, and autoradiographed. The blot was probed with a 2271-bp PCR product amplified with the paired primers Tena36F2/Tena43R.
Gels were dried, and radiolabeled DNA--protein complexes were autoradiographed using a PhosphorImager (Molecular Dynamics, Sunnyvale, CA).
After rinsing in running tap water, the autoradiographed sections were stained with toluidine blue.
The membrane was washed twice with SSPE buffer (150 mmol/L sodium chloride, 10 mmol/L sodium biphosphate,1 mmol/L EDTA) containing 10 g/L sodium dodecyl sulfate at 55[degrees]C and autoradiographed for 6 days at -70'C using Hyper film MP (Amersham Pharmacia Biotech.) and an intensifying screen.
Sections of 1-1.5 [micro]m were cut and autoradiographed at 4[degrees]C in Ilford L-4 emulsion (Ferranti-Dege).
Gels were dried and autoradiographed for 1 day at room temperature.
Blots were washed three times in 1X SSC (prepared from a 20X SSC stock solution: 3.0 M sodium chloride, 0.3 M sodium citrate, pH = 7.0) and 0.1% SDS at 65 [degrees] C 5 min each, followed by two additional washes in 0.1 X SSC and 0.1% SDS (65 [degrees] C) 15 min each, and autoradiographed with Kodak XAR film.