Background: Amyloid-[sz] deposition and accumulation of autophagic vacuoles
are pathologic features of Alzheimer's disease (AD).
The numbers and sizes of these autophagic vacuoles
were equivalent in wild-type and Atg5-deficient MEFs (Fig.
But autophagic vacuoles
were also detected in both groups of cells in addition to clear apoptotic cell death sparks in our TEM micrographs.
were detected by monodansylcadaverine (MDC) staining.
Autophagosomes, also referred to as initial autophagic vacuoles
(AVi), were typically identified as vacuolar structures containing cellular contents that appeared similar to the cytosol and organelles in the cell, as presented in Figure 1(a).
Indeed, we observed a larger population of autophagic vacuoles
that engulfed mitochondria in MDA-MB-231 cells than in MCF-10A cells by electron microscopic study (Figures 4(c) and 4(d) and supplemental Figure 2).
8,9) It involves degradation of cellular components within the dying cell in autophagic vacuoles
and is not inflammatory.
It has been reported that chronic administration of efavirenz in adult Wistar rats resulted in some cellular degenerative changes like sparse cellular population, pyknotic nuclei with some microcystic changes, autophagic vacuoles
and vacuolations in the stroma of the treated superior colliculus and lateral geniculate body as compared to the control group (Adjene et al.
In contrast, generalized edema (c-f) dispersed chromatin (c,f), swollen mitochondria (c-f) and autophagic vacuoles
(e) were distinguished giving the appearance of necrotic cells, particularly in d and e.
Endoplasmic reticulum, a small Golgi complex and some large cytoplasmic vacuoles were found, which possibly represent autophagic vacuoles
, as suggested by the membranous whorls within the vacuoles (Figure 3B and D).
Large autophagic vacuoles
may not be visible on biopsy unless the patient has been treated with the drug for at least 6 months.
were formed and the prefollicular epithelium was partly destroyed and folded on itself.