Moreover, we have fund that EMS horses suffer for reduced mitochondrial biogenesis, limited fusion, and abundance of autophagosomes and autolysosomes
formation that affects their "sternness.
Autophagic flux was calculated as the difference between LC3-II levels in the absence (0 hr) and presence (4 hr) of autolysosome
inhibitors bafilomycin A1 (0.
These sections revealed structures within the dinoflagellate cells that appeared in TEM preparations to correspond with dinoflagellate autolysosomes
(Schmitter, 1971; Schmitter and Jurkiewicz, 1981).
7) Moreover, all stages of autophagosome biogenesis were observed in Atg5-deficient MEFs, including formation of isolation membranes (membrane cisternae curving around a part of the cytoplasm), autophagosomes (double-membrane vacuoles generated by sealing of the edges of the isolation membrane), and autolysosomes
(single-membrane vacuoles generated by fusion of an autophagosome and lysosome).
Some lamellated phagolysosomes or autolysosomes
were also seen with variably electron-dense material.
Electron microscopy revealed formation of autophagosomes and autolysosomes
in HL-60bcl-2 cells after exposure to VK2.
2004a, b) argue from ultrastructural evidence that NPs control their own reduction in size by becoming autophagic through the action of "cell-size-reducing autolysosomes
LC3 turnover assay is one of the primary methods to evaluate autophagic flux, which is based on the observation that LC3-II is degraded in autolysosomes
Deretic, "Autophagosomes, phagosomes, autolysosomes
, phagolysosomes, autophagolysosomes .
Exocytosis of autolysosomes
as a mechanism of TFEB-mediated cellular clearance in Pompe disease," Autophagy, vol.
The substrate (cargo) for autophagy is degraded by lysosomal acid hydrolases, including cathepsins D, B, and L, after the fusion of lysosomes and autophagosomes to form autolysosomes
As shown in Figures 4(a) and 4(b), compared with the PSB control, 3-MA treatment resulted in a decrease of number of autolysosomes
and autophagy vacuoles.