Bleaching responses were analyzed as the magnitude of the difference in chlorophyll autofluorescence
between a treated anemone and a size-matched control anemone of the same strain ([DELTA]F).
VELscope which has been recently introduced utilizes the property of autofluorescence
to help in the diagnosis of dysplastic changes.
To assess the influence of age and tested measurements on the outcome variable skin autofluorescence
, the stepwise multiple regression analysis was performed.
Microphotographs were taken by a camera in the bright field under UV to reveal autofluorescence
and nuclei stained by DAPI and under appropriate filter to reveal oxidized H2DCFDA or DHE fluorescence.
The comparison of skin autofluorescence
intensity image and image of autofluorescence
photobleaching rates, both extracted from the skin video at UV-illumination, is illustrated at Fig.
0, revision 4, Molecular Devices, Sunnyvale, California) as follows: (1) SHG (360-400 nm, color-coded red), a nonlinear scattering signal originating from tissue collagen; (2) short wavelength autofluorescence
(420-490 nm, color-coded green), originating in part from reduced nicotinamide adenine dinucleotide and flavin adenine dinucleotide in normal epithelial, neoplastic, and inflammatory cells, and from elastin in the alveolar septa; and (3) long wavelength autofluorescence
(550-650 nm, color-coded blue), originating in part from carbon-laden macrophages.
Thus concluding the sensitivity of autofluorescence
Rats not injected with GFP were injected with saline and euthanized for autofluorescence
A positive cytology diagnosis included cytomorphological features consistent with mycobacterial infection and confirmation of the organism on Ziehl-Neelsen stain or Papanicolaou autofluorescence
The low analytical sensitivity of dye-conjugated antibodies can, in part, be attributed to high background in the visible region caused by autofluorescence
of the membrane and sample matrix.
The control treatment for the 12-hour immersion showed no marks, whilst the osmotic induction control exhibited autofluorescence
in all three structures (Figs.
Many marine organisms exhibit autofluorescence
; therefore, this raises the question whether autofluorescence
also occurs in abalone and whether it could hinder fluorescence studies.