auramine-rhodamine stain


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auramine-rhodamine stain

a fluorescent dye consisting of auramine O, rhodamine B, and phenol that is used in the fluorochrome acid-fast staining method. The dye binds to mycolic acids in the cell wall of bacteria and resists decolorization with acid alcohol.
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By Ziehl-Neelsen stain AFB stain pink curve/straight beaded rods against blue background by Auramine-Rhodamine stain AFB appears as bright reddish-yellow fluorescing rods against a dark background and by Papanicolaou stain fluorescent bacilli appeared as slender, often beaded, yellow-green, straight or slightly curved rods of relatively uniform length against a dark background.
Not to mention names, but his famous father was the developer/discoverer/inventor of the auramine-rhodamine stain, also known as Truant's auramine-rhodamine stain.
One important consideration is that the organisms may fail to fluoresce or may fluoresce only weakly by auramine-rhodamine stain.