The present study was conducted with an objective of comparing the efficacy of various counter stains in Auramine O staining method enabling better detection of acid fast bacilli in sputum smears.
The different reagents used in Auramine O staining technique were 0.3% Auramine O , 1% acid alcohol as decolorizer and various counter stains like 0.1% potassium permanganate, 0.1% methylene blue, 0.1% toluidine blue, 0.1% malachite green and undiluted blue ink (9).
This study highlights on improvising the already established Auramine O staining method with minor modifications in counter staining and evaluating the efficacy of these counter stains.
Standard fluorescence staining method using Auramine O stain was introduced in 1978.
In the present study, we compared the efficacy of five different counter stains, all of them in a concentration of 0.1%, in Auramine O method using Light-Emitting Diode Fluorescence Microscopy.
Kinyoun and Fluorescent staining using Auramine O and Rhodamine B are better staining techniques in the detection of AFB than Ziehl Neelsen Staining.
Slides were stained with routine ZN, Kinyoun and fluorescent staining technique using Auramine O and Rhodamine B to demonstrate the presence of acid fast bacilli in tissue.
Slides were stained with routine ZN staining, Kinyoun staining and fluorescent staining technique using Auramine O and Rhodamine B to demonstrate the acid fast bacilli in tissue.
They used the Kuper and May (1960) method by using Auramine O and Rhodamine B.8 Study conducted by Greenwood and Fox in 1973 on "A comparison of methods for staining tubercle bacilli in histological sections" showed 42 (60%) positive AFB on fluorescent technique out of 70 tuberculosis cases.