atrogin-1

atrogin-1

An E3 ubiquitin ligase expressed in cardiac and skeletal muscle that directs muscle protein degradation after the muscle atrophy response has been triggered by Akt1-induced phosphorylation of FOXO, a transcription factor which also activates ubiquitin ligases and MuRF-1. Atrogin-deficient mice are resistant to muscle atrophy.
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These responses were associated with upregulation of Atrogin-1 in both muscles and C2C12 cells, and LC3 gene only in soleus.
O TF e um importante regulador de marcadores anabolicos e catabolicos e a pratica regular dessa atividade pode contribuir para a fosforilacao de alguns marcadores anabolicos como a via Akt/mTOR e na cascata de sinalizadores que culminam na sintese proteica como a GSK-3beta, p70S6K e 4E-BP1, alem da inibicao de marcadores catabolicos como Foxo1, Foxo3, atrogin-1 e MuRF-1 (Leger e colaboradores, 2006; Terzis e colaboradores, 2008).
Simultaneously intense exercise may stimulate the ubiquitin proteasome pathway and the induction of muscle-specific E3-ubiquitin ligases, atrogin-1 and muscle RING finger-1 (MuRF1) which promote muscle turnover (Rahbek et al.
Similarly, atrogin-1 expression was significantly increased in skeletal muscle at end-stage disease in CBA-administered, SIV-infected macaques (Molina et al.
NF-[kappa]B activation leads to increased expression of the E3 ubiquitin ligases MuRF-1 and Atrogin-1 [153] and muscle proteolysis, leading to atrophy.
Methods: TGF-[beta]1 and atrogin-1 expression was evaluated by RT-qPCR and/or ELISA; Smad3 phosphorylation by western blot; Smad4 nuclear translocation by indirect immunofluorescence; and ROS levels by DCF probe fluorescent measurements.
Atrogin-1, a muscle-specific Fbox protein highly expressed during muscle atrophy.
Briefly, IGF-1 upregulates multiple intracellular signals that inhibit the secretion of several ubiquitin-proteins, including atrogin-1, FOX-1 and MuRF1, that have been suggested as primary factors evoking skeletal muscle atrophy [1].
There was no age-related difference in expression of atrogin-1.
Atrogin: Protein expressed during muscle atrophy; atrogin-1 is expressed in cardiac and skeletal muscle and directs muscle protein degradation after muscle atrophy response has been triggered.
Taq-Man quantitative real-time PCR reactions were performed in triplicate, using an Eco Real-Time PCR System (Illumina, USA) with predesigned primer sets for mouse atrogin-1, MuRF-1, and the housekeeping gene 18S (TaqMan Assays-on-Demand, Applied Biosystems, USA).
The mRNA expression of mechanogrowth factor (MGF), mammalian target of rapamycin (mTOR), atrogin-1, and muscle RING finger-1 (MuRF-1) were quantified using real-time polymerase chain reaction on the basis of current methodology (Bustin et al.