Rouach, "How do astrocytes
shape synaptic transmission?
, seeded in 96-well plates, were stimulated with 1 [micro]g/ml LPS for 24 h with or without isoflavones.
: Bidirectional Control of Blood Flow and Neuronal Function
are major producers of these cytokines within the CNS, we asked whether irisin regulated the release of cytokines from astrocytes
during its protective effects on neurons.
(14) Mice deficient for the genes in this pathway (Lif, gp130, Lifr, or Stat3) show impairment of astrocytic differentiation of NSCs in vivo, indicating that JAK-STAT3 signaling is indispensable for the production of astrocytes
during brain develop ment.12)-14)-16)
In the new study, Sejnowski's team looked for the first time at the longer-term memory of mice with disrupted astrocytes
. They used genetically engineered animals lacking a receptor called type 2 inositol 1,4,5-trisphosphate (IP3R2), which astrocytes
rely on to release calcium for communication.
In the brain, astrocytes
are the support cells that act as guides to direct new cells, promote chemical signaling, and clean up byproducts of brain cell metabolism.
They observed that the level of expression of this gene declines naturally, a short time before astrocyte
Thus, surprisingly, nestin does not control the generation of neurons by acting within neural stem cells, but indirectly by regulating the neurogenesis-inhibitory Notch signals that neural stem cells receive from astrocytes
, important constituents of the neurogenic niche.
Primary adult human astrocytes
and endothelial cells (Angio-Proteomie, Boston, MA) were established on plates that were initially coated with 0.2% porcine skin gelatin at room temperature.
In cells grown from people with Alzheimer's disease and in mouse astrocytes
engineered to carry a human Alzheimer's disease gene variant, the amount of NHE6 was about half the amount found in normal cells.