Treatment consisted of eliminating leaves with more than 50% of symptoms, while those with less than 50% of symptoms, only the affected part was eliminated and 16% of leaf number 3 was also eliminated (from top to low), even asymptomatic, thus allowing the elimination of potentially infectious foliar tissue, avoiding the production and release of fungal ascospores
We found that these locations had the greatest probability of high levels of overwintering inoculum and serve as "canaries" for when ascospore
release does occur.
nidulans in clinical microbiology laboratories is commonly based on the characteristic microscopic morphology, the production of hulle cells, or the production of ascospores
Fifty microconidia, macroconidia or chlamydospores were measured in each monoconidial culture, while in each monoascosporic isolate 50 macroconidia, 25 perithecia, and 50 ascospores
After fertilization of the stromata, perithecia with asci and ascospores
(100 ul of 1 x 106 spore ml-1) was inoculated in the centre of plates with PDA medium using a micro applicator and was spread to cover the whole plate.
a) the average ascospore
volume in tetrasporic asci: 15,000 um3 instead of 10,000 um3 and 5,000 um3, the last two measured in the single last two Tuber species, respectively; b) the ascospores
shape and ornamentation: spherical (22.5-55 um), rarely sub-spherical, enclosed by a reticulate-alveolate exosporium with net meshes delimited by 4-6 walls 5-7.5 um high; c) a peridium constituted by very small and flat warts, almost never transversely striated; d) the odour, at first phenolic (3-methylanisole), then grateful due to the presence of ethyl-methyl-chetone, without any phenolic component; e) thecolour of the gleba, light-brown with numerous, irregularly flexuous and subtle sterile veins; f) theovoid (77-95 x 7-115 um) asci, supported by a hooked pedicle and containing 1-6 spores.
The first stage of the disease can overwinter either as cleistothecia are fruiting bodies that produce ascospores
. These structures form on infected leaves late in the growing season and wash down onto the trunks and spurs of the vine with winter rains.
On the basis of a study of coprophilous fungi from Zulia state, Venezuela, one specie of the family Coniochaetaceae, with cleistothecial ascocarp and ascospores
with germ pore was isolated from dove dung.
were hyaline, one-celled, and lenticular, with two closely opressed equatorial crests.